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OP44

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Anti-APC (Ab-1) Mouse mAb (FE9)

liquid, clone FE9, Calbiochem®

Synonym(e):

Anti-Adenomatous Polyposis Coli

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.43

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

FE9, monoclonal

Form

liquid

Enthält

≤0.1% sodium azide as preservative

Speziesreaktivität

rat, human, mouse

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

do not freeze

Isotyp

IgG1

Versandbedingung

wet ice

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... APC(324)

Allgemeine Beschreibung

Anti-APC (Ab-1), mouse monoclonal, clone FE9, recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells. It is validated for Western botting.
Protein G purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP40 cells. Recognizes the ~300 kDa APC protein as well as a variety of truncated forms.
Recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells.
  • Antibody Target Gene Symbol: APC
  • Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC
  • Entrez Gene Name: adenomatous polyposis coli
  • Hu Entrez ID: 324 (Related Antibodies: OP80, ST1150, OP62, OP47L)
  • Mu Entrez ID: 11789
  • Rat Entrez ID: 24205
  • Immunogen

    a synthetic peptide corresponding to the N-terminal 35 amino acids of APC

    Anwendung

    Immunoblotting (1 µg/ml, see comments)

    Verpackung

    Please refer to vial label for lot-specific concentration.

    Warnhinweis

    Toxicity: Standard Handling (A)

    Physikalische Form

    In 50 mM sodium phosphate buffer, pH 7.5, 0.2% gelatin.

    Hinweis zur Analyse

    Positive Control
    HCT116 cells for p300, SW480 cells for truncated APC (p147)

    Sonstige Hinweise

    Koetsier, P. A., et al. 1993. BioTechniques15, 258.
    Smith, K. J., et al. 1993. Proc. Natl. Acad. Sci., USA90, 2846.
    Su, L.-K., et al. 1993. Can. Res.53, 2728.
    Boynton, R. F., et al. 1992. Proc. Natl. Acad. Sci. USA89, 3385.
    D′Amico, D., et al. 1992. Cancer Res.52, 1996.
    Fearon, E. R., and Jones, P. A., 1992. FASEB J.6, 2783.
    Miyoshi, Y., et al. 1992. Proc. Natl. Acad. Sci. USA89, 4452.
    Powell, S. M., et al. 1992. Nature359, 235.
    Groden, J., et al. 1991. Cell66, 589.
    Kinzler, K. W., et al. 1991. Science253, 661.
    Nishisho, I., et al. 1991. Science253, 665.

    Rechtliche Hinweise

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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    Lagerklassenschlüssel

    11 - Combustible Solids

    WGK

    WGK 1

    Flammpunkt (°F)

    Not applicable

    Flammpunkt (°C)

    Not applicable


    Analysenzertifikate (COA)

    Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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    Mireia Menéndez et al.
    Gastroenterology, 134(1), 56-64 (2008-01-02)
    We identified the APC N1026S variant of unknown malignant potential in the adenomatous polyposis coli (APC) gene in a Spanish attenuated familial adenomatous polyposis (AFAP) family. The variant was located in the first of the 4 highly conserved 15-amino acid
    Jason L Larabee et al.
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    Claudia Gaspar et al.
    PLoS genetics, 5(7), e1000547-e1000547 (2009-07-07)
    Germline mutations in the adenomatous polyposis coli (APC) gene are responsible for familial adenomatous polyposis (FAP), an autosomal dominant hereditary predisposition to the development of multiple colorectal adenomas and of a broad spectrum of extra-intestinal tumors. Moreover, somatic APC mutations

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