NA19L

Anti-Replication Protein A (Ab-3) Mouse mAb (RPA34-20)

• Synonym(e): Anti-RP-A

Eigenschaften

• Antikörperform: purified antibody
• Qualitätsniveau: 100
• Klon: RPA34-20, monoclonal
• Form: lyophilized
• Speziesreaktivität: yeast, human, mouse
• Isotyp: IgG2a

Beschreibung

Allgemeine Beschreibung

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the complexed (phosphorylated) and the ~34 kDa unphosphorylated subunit of replication protein A.

Recognizes the complexed (phosphorylated) and unphosphorylated subunits of replication protein A in HeLa and U293 cells and colon carcinoma tissue.

The replication system of a cell contains several essential components among which are replication factor A (RFA, RPA, or HeLa cell SSB), PCNA, and replication factor C (RFC) as well as several other proteins. RPA, a multi-subunit protein containing a 70 kDa, 34 kDa, and 11 kDa protein, may play a critical regulatory role in replication based on its functioning during both the initiation and elongation stages of DNA replication. The protein is highly conserved, having been identified in a range of species from yeast (Saccharomyces cerevisiae) to humans. The primary function of RPA appears to be as an auxiliary protein for both DNA polymerase α and δ. RPA undergoes a cell cycle dependent phosphorylation, which is restricted to the p34 protein. In addition to existing as part of the multi-subunit structure, p34 has also been identified as being present in a free, unphosphorylated form. Recent data shows that RPA is restricted to the nucleus and is overexpressed in transformed cells as well as in several tumor cells.

Immunogen

Epitope: within the p34 subunit of replication protein A

Human

replication protein A purified from U293 cells

Anwendung

Immunoblotting (5 μg/ml)
Immunofluorescence (2.5 μg/ml)
Immunoprecipitation (1 μg/reaction)
Paraffin Sections (2.5 μg/ml, heat pre-treatment required)

Physikalische Form

Lyophilized from 20 mM ammonium bicarbonate solution, 100 μg BSA.

Rekonstituierung

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 μg/ml; product will be more stable

Hinweis zur Analyse

Positive Control
HeLa or U293 cells or colon carcinoma tissue

Sonstige Hinweise

Replication protein A (Ab-3) will react with both the complexed (phosphorylated) form of the protein as well as the free (unphosphorylated) 34 kDa subunit. Replication protein A can be used as a marker for cell division. Antibody should be titrated for op

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable