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371720

Sigma-Aldrich

Anti-Giα-1-Subunit, Internal (159-168) Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

diluted serum

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

liquid

Enthält nicht

preservative

Speziesreaktivität

mouse

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

OK to freeze
avoid repeated freeze/thaw cycles

Isotyp

IgG

Versandbedingung

wet ice

Lagertemp.

−70°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

mouse ... Gnai1(14677)

Allgemeine Beschreibung

Anti-Giα-1-Subunit, Internal (159-168), rabbit polyclonal, recognizes Giα-1 subunit. Does not cross-react with Giα-2, Giα-3, Goα, or Gsα subunits. It is validated for use in Western blotting.
Rabbit polyclonal antibody supplied as diluted serum. Recognizes Giα-1 subunit.
Recognizes Giα-1 subunit. Does not cross-react with Giα-2, Giα-3, Goα, or Gsα subunits.

Immunogen

a synthetic peptide (LDRIAQPNYI) corresponding to amino acids 159-168 of mouse Giα-1, conjugated to KLH

Anwendung

Immunoblotting (1:1000)

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Serum diluted in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.

Rekonstituierung

Following initial thaw, aliquot and freeze (-70°C).

Sonstige Hinweise

Does not cross-react with Giα-2, Giα-3, Goα, or Gsα subunits. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Giα-1, Giα-2, Giα-3, Goα and Gsα. Variables associated with assay conditions will dictate the proper working dilution.
Kumar, R., et al. 1994. J. Mol. Cell. Cardiol.26, 1537.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.
Goldsmith, P.P., et al. 1987. J. Biol. Chem.262, 14683.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Hiroko Ohtsuka et al.
PloS one, 12(1), e0169609-e0169609 (2017-01-11)
Stromal cell-derived factor-1α (SDF-1α)-induced platelet aggregation is mediated through its G protein-coupled receptor CXCR4 and phosphatidylinositol 3 kinase (PI3K). Here, we demonstrate that SDF-1α induces phosphorylation of Akt at Thr308 and Ser473 in human platelets. SDF-1α-induced platelet aggregation and Akt
H-D Yan et al.
British journal of pharmacology, 139(3), 605-615 (2003-06-06)
(1) The effects on the whole-cell carbachol-induced muscarinic cationic current (mIcat) of antibodies against the alpha-subunits of various G proteins, as well as the effect of a Gbetagamma subunit, were studied in single guinea-pig ileal smooth muscle cells voltage-clamped at
Yatin M Patel et al.
Blood, 101(12), 4828-4835 (2003-03-01)
We have examined platelet functional responses and characterized a novel signaling defect in the platelets of a patient suffering from a chronic bleeding disorder. Platelet aggregation responses stimulated by weak agonists such as adenosine diphosphate (ADP) and adrenaline were severely
Takashi Hirakawa et al.
Endocrinology, 144(9), 3872-3878 (2003-08-23)
Using a Leydig tumor cell line (MA-10) transiently transfected with the human lutropin receptor (hLHR) and mutants thereof, we examined the identity of the G proteins activated by the agonist-engaged hLHR-wild type (wt) and by three of its naturally occurring
Shannon M Gifford et al.
The Journal of endocrinology, 190(2), 373-384 (2006-08-11)
Uterine artery endothelial cells (UAEC) derived from pregnant (P-UAEC) and nonpregnant (NP-UAEC) ewes retain pregnancy-specific differences in cell signaling as well as vasodilator production through passage 4. In particular, when P- and NP-UAEC are stimulated with ATP over a 2.5

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