The specific isoform is not identified. The SDS PAGE reveals a molecular weight of 47 kDa for this product. However, this molecular weight includes a C-terminal 6His tag (0.8 kDa) and an N-terminal GST tag (approx. 26 kDa). The molecular weight of the remaining recombinant protein is just over 20 kDa. See the link below to review the product datasheet, which includes the sequence:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/610/13-173.pdf
13-173
MBP, recombinant
Synonym(e):
myelin basic protein
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500 μG
CHF 419.00
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500 μG
CHF 419.00
About This Item
UNSPSC-Code:
12352200
eCl@ss:
32160405
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
bovine
Qualitätsniveau
Form
liquid
Mol-Gew.
Mw ~47 kDa
Hersteller/Markenname
Upstate®
Konzentration
1 mg/mL
Methode(n)
activity assay: suitable
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Allgemeine Beschreibung
Product Source: Recombinant fusion protein containing bovine myelin basic protein (MBP) with an N-terminal GST tag and a C-terminal 6His tag, expressed in E. coli.
Qualität
Routinely evaluated by kinase assay
Physikalische Form
ProBond Resin chromatography
500 µg recombinant MBP in 500 µl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 500 mM imidazole, 1 mM PMSF, and 10% glycerol. Frozen solution.
Lagerung und Haltbarkeit
Stable for 2 years at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Hinweis zur Analyse
Specific Activity: This lot was successfully phosphorylated in a non-radioactive kinase assay using recombinant active MAP Kinase 2/Erk2 (Catalog # 14-173). MBP phosphorylation was detected by immunoblot analysis using anti-phospho-MBP (Catalog # 05-429). Immunoblot shown to the right.
Sonstige Hinweise
Purity information is lot specific, please consult the certificate of analysis
Rechtliche Hinweise
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Vanessa D Stewart et al.
FEBS letters, 596(22), 2952-2973 (2022-09-15)
Myelin-associated glycoprotein (MAG) and Nogo inhibit neurite outgrowth by binding to receptors such as NgR1, PirB and LRP1, and they have also been shown to induce phosphorylation of Smad2, a key intermediate in the transforming growth factor β (TGFβ) signalling
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Which MBP isoform is product 13-173 (SKU)? Can I please have the sequence of it?
1 answer-
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