Accéder au contenu
Merck

Isoproterenol-Induced Permeability Transition Pore-Related Dysfunction of Heart Mitochondria Is Attenuated by Astaxanthin.

Biomedicines (2020-10-24)
Roman Krestinin, Yulia Baburina, Irina Odinokova, Alexey Kruglov, Irina Fadeeva, Alena Zvyagina, Linda Sotnikova, Olga Krestinina
RÉSUMÉ

Mitochondria are key organelles of the cell because their main function is the capture of energy-rich substrates from the cytoplasm and oxidative cleavage with the generation of carbon dioxide and water, processes that are coupled with the synthesis of ATP. Mitochondria are subject to oxidative stress through the formation of the mitochondrial permeability transition pore (mPTP). Various antioxidants are used to reduce damage caused by oxidative stress and to improve the protection of the antioxidant system. Astaxanthin (AST) is considered to be a dietary antioxidant, which is able to reduce oxidative stress and enhance the antioxidant defense system. In the present investigation, the effect of AST on the functional state of rat heart mitochondria impaired by isoproterenol (ISO) under mPTP functioning was examined. It was found that AST raised mitochondrial respiration, the Ca2+ retention capacity (CRC), and the rate of TPP+ influx in rat heart mitochondria (RHM) isolated from ISO-injected rats. However, the level of reactive oxygen species (ROS) production increased. In addition, the concentrations of cardiolipin (CL), Mn-SOD2, and the proteins regulating mPTP rose after the injection of ISO in RHM pretreated with AST. Based on the data obtained, we suggest that AST has a protective effect in rat heart mitochondria.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Saccharose, ≥99.5% (GC), BioXtra
Sigma-Aldrich
Antimycine A from Streptomyces sp.
Sigma-Aldrich
Albumine de sérum bovin, lyophilized powder, essentially fatty acid free, ≥96% (agarose gel electrophoresis)
Sigma-Aldrich
Oligomycine A, ≥99% (HPLC)
Sigma-Aldrich
Acide éthylène glycol-bis(2-aminoéthyléther)-N,N,N′,N′-tétraacétique, for molecular biology, ≥97.0%
Sigma-Aldrich
Peroxydase from horseradish, Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid (using pyrogallol)
Sigma-Aldrich
Menadione, crystalline
Sigma-Aldrich
Ethylenediaminetetraacetic acid, ACS reagent, 99.4-100.6%, powder
Sigma-Aldrich
D-Mannitol, ≥98% (GC)
Sigma-Aldrich
Superoxide Dismutase from bovine erythrocytes, lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret
Sigma-Aldrich
Acridine Orange 10-nonyl bromide