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GE28-9537-66

HiTrap® TALON® Crude

Cytiva 28-9537-66, pack of 5 × 1 mL

Synonyme(s) :

Crude TALON Column, HiTrap TALON, TALON Crude Column

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About This Item

Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56

ligand

TALON® Superflow Cobalt-IMAC

Conditionnement

pack of 5 × 1 mL

Fabricant/nom de marque

Cytiva 28-9537-66

Paramètres

1 ml/min - 4 ml/min flow rate
3 bar

D.I. de la colonne

7 mm

Matrice

6% cross-linked agarose

Taille des particules

60-160 μm

cleaning

2-14(Co2+-stripped medium)

working range

3-12(Co2+-stripped medium)

Capacité

≥20 mg binding capacity (histidine-tagged protein)

Température de stockage

2-8°C

Catégories apparentées

Description générale

HiTrap® Talon® crude 1 mL and 5 mL columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Application

Talon® Superflow is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Talon® Superflow and prepacked HiTrap® Talon® crude columns allow Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

HiTrap® Talon® crude columns allow for rapid separations and easy scale-up. Samples can be applied directly to the column after thorough cell disruption - without precentrifugation and filtration. The filters in the top and bottom of the column make it possible to directly load unclarified lysates on the column without causing back pressure problems or leakage of the Talon® Superflow beads. Prior to loading samples on HiTrap® Talon® crude columns, cell lysate should be prepared using methods such as sonication, homogenization, or freeze/thaw.

Caractéristiques et avantages

  • Columns prepacked with cobalt-based chromatography media designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
  • Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
  • Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
  • Prepacked HiTrap® Talon® crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of Proteins.
  • HiTrap® Talon® crude columns allow repeated runs without stripping, cleaning, or Co2+ recharging.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

HiTrap is a registered trademark of Cytiva
Talon is a registered trademark of Clontech

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

FlameFlame
GHS02,GHS02

Mention d'avertissement

Warning-Warning

Mentions de danger

H226 - H226

Classification des risques

Flam. Liq. 3

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

100.4 - 109.4 °F

Point d'éclair (°C)

38 - 43 °C

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

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Protocoles

Purification of Histidine-Tagged Proteins using TALON® Superflow

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

Handling Inclusion Bodies in Recombinant Protein Expression

How to isolate proteins from inclusion bodies using Cytiva products.

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