16-222
Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate
clone 3H10, Upstate®, from mouse
Synonyme(s) :
H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
FITC conjugate
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
3H10, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Upstate®
Technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
Isotype
IgG
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
phosphorylation (pSer10)
Informations sur le gène
human ... HIST1H3F(8968)
Description générale
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Spécificité
Broad species cross-reactivity is expected.
Recognizes histone H3 phosphorylated at Ser10, MW 17 kDa.
Immunogène
A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3. Clone 3H10.
Epitope: Ser10
Application
Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate is a Mouse Monoclonal for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p,is labeled with Fluorescein isothiocyanate (FITC), published in peer reviewed journals & validated in WB, ICC & IF.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Western Blot Analysis: 0.5-5 µg/mL of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid.
Qualité
Immunocytochemistry: Mitotic HeLa cells showed positive chromosome staining with 4μg/mL of this antibody.
Description de la cible
17 kDa
Forme physique
Protein G Purified
Purified mouse monoclonal IgG in buffer containing PBS with 0.05% sodium azide, pH7.1.
Stockage et stabilité
Stable for 1 year at from date of receipt.
Remarque sur l'analyse
Control
Mitotic HeLa cells (IF).
Mitotic HeLa cells (IF).
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Tumor-suppressing function of caspase-2 requires catalytic site Cys-320 and site Ser-139 in mice.
Keqin Ren,Jing Lu,Aleksey Porollo,Chunying Du
The Journal of Biological Chemistry null
Antje M Wengner et al.
Molecular cancer therapeutics, 15(4), 583-592 (2016-02-03)
Monopolar spindle 1 (Mps1) has been shown to function as the key kinase that activates the spindle assembly checkpoint (SAC) to secure proper distribution of chromosomes to daughter cells. Here, we report the structure and functional characterization of two novel
Agata Zieba et al.
Molecular & cellular proteomics : MCP, 11(7), M111-M111 (2012-03-24)
Fundamental open questions in signal transduction remain concerning the sequence and distribution of molecular signaling events among individual cells. In this work, we have characterized the intercellular variability of transforming growth factor β-induced Smad interactions, providing essential information about TGF-β
Christopher Merritt et al.
Development (Cambridge, England), 137(11), 1787-1798 (2010-05-01)
FBF-1 and FBF-2 (collectively FBF) are two nearly identical Puf-domain RNA-binding proteins that regulate the switch from mitosis to meiosis in the C. elegans germline. In germline stem cells, FBF prevents premature meiotic entry by inhibiting the expression of meiotic
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