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H9789

Sigma-Aldrich

Anti-HMGB2 (HMG2) antibody produced in rabbit

affinity isolated antibody

Synonyme(s) :

Anti-High mobility group box 2, Anti-High mobility group protein 2

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

rat, human, mouse

Conditionnement

antibody small pack of 25 μL

Technique(s)

immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates.
indirect immunofluorescence: 1-2 μg/mL using fixed PC12 cultured cells.
western blot: 2-4 μg/mL using PC12 whole cell lysates.

Numéro d'accès UniProt

P26583

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... HMGB2(3148)
mouse ... Hmgb2(97165)
rat ... Hmgb2(29395)

Description générale

The high mobility group (HMG) proteins are subdivided into 3 families based on their functional sequence motif characteristic: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) and the HMGA (formerly HMG-I/Y/C) family. The HMGB protein family, including HMGB1, HMGB2, and HMGB3, are highly conserved (more than 80% amino acid identity), and indistinguishable in their biochemical properties. The high-mobility group box 2 (HMGB2) is a ~25 kDa protein of 209 amino acids that consists of two homologous HMG-boxes rich in basic amino acids and an acidic tail at the carboxy-terminus. HMGB2 is restricted mainly to lymphoid organs and testes, although it is widely expressed in embryogenesis.

Immunogène

synthetic peptide corresponding to amino acid 165-181 of human HMGB2, conjugated to KLH. The corresponding sequence is conserved in mouse and differs by one amino acid in rat.

Application

Anti-HMGB2 (HMG2) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol.

Actions biochimiques/physiologiques

The high-mobility group box 1 and 2 (HMGB 1 & 2) proteins participate in the regulation of chromatin structure as well as being involved in transcription regulation, DNA repair, recombination, differentiation and extracellular signalling.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

The dual role and therapeutic potential of high-mobility group box 1 in cancer
He SJ, et al.
Testing, 8(38), 64534-64534 (2017)
Yasunari Yamanaka et al.
Cell reports, 11(6), 967-976 (2015-05-06)
Long non-coding RNAs (lncRNAs), including natural antisense transcripts (NATs), are expressed more extensively than previously anticipated and have widespread roles in regulating gene expression. Nevertheless, the molecular mechanisms of action of the majority of NATs remain largely unknown. Here, we
Antisense RNA controls LRP1 Sense transcript expression through interaction with a chromatin-associated protein, HMGB2
Yamanaka Y, et al.
Testing, 11(6), 967-976 (2015)
Yu Hu He et al.
Arteriosclerosis, thrombosis, and vascular biology, 37(4), 717-729 (2017-02-12)
In a previous study, we established diabetic and nondiabetic minipig models with coronary artery in-stent restenosis (ISR). Mass spectrometry showed that high-mobility group box (HMGB) 2 level was higher in ISR than in non-ISR tissue from diabetic minipigs. We here
Reduced fertility and spermatogenesis defects in mice lacking chromosomal protein Hmgb2
Ronfani L, et al.
Development, 128(8), 1265-1273 (2001)

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