Our lab has not conducted testing on the inhibition of P8465 on PreScission Protease. However, considering that the protein structure of PreScission Protease contains a cysteine nucleophile in its active site and the protein folding resembles that of chymotrypsin-like serine protease, it is likely that the inhibition is caused by ABESF and/or E-64, both of which are included in the cocktail of P8465.
P8465
Protease Inhibitor Cocktail
lyophilized powder, for the inhibition of serine, cysteine, aspartic, metalloproteases and aminopeptidases, for use with bacterial cell extracts, lyophilized powder
Synonyme(s) :
Protease inhibitor
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5 ML
121,00 $
25 ML
482,00 $
121,00 $
Disponible pour expédition le02 avril 2025Détails
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5 ML
121,00 $
25 ML
482,00 $
About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77
121,00 $
Disponible pour expédition le02 avril 2025Détails
Produits recommandés
Nom du produit
Protease Inhibitor Cocktail powder, for use with bacterial cell extracts, lyophilized powder
Niveau de qualité
Forme
lyophilized powder
Solubilité
water: soluble
Température de stockage
−20°C
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Catégories apparentées
Description générale
The Protease Inhibitor Cocktail is a lyophilized powder for use in the inhibition of proteases present in bacterial cell extracts.
The product contains individual components that target serine, cysteine, aspartic, and metalloproteases, as well as aminopeptidases.
The product contains individual components that target serine, cysteine, aspartic, and metalloproteases, as well as aminopeptidases.
Application
The protease inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been optimized and tested for bacterial cell use, with broad specificity against serine, cysteine, and aspartic proteases, metalloproteases, and aminopeptidases.
Actions biochimiques/physiologiques
This mixture contains individual components, including AEBSF at 23 mM, EDTA at 100 mM, Bestatin at 2 mM, Pepstatin A at 0.3 mM, and E-64 at 0.3 mM. AEBSF acts to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Pepstatin A inhibits acid proteases. EDTA is an inhibitor of metalloproteases.
Caractéristiques et avantages
Broad specificity against serine, cysteine, aspartic, and metalloproteases, as well as aminopeptidases.
Contains individual components, including AEBSF, EDTA, Bestatin, Pepstatin A, and E-64, each targeting specific types of proteases.
Lyophilized powder is stable for at least 2 years when stored unopened at -20°C.
Supplied with a vial of DMSO for preparation of a cocktail solution.
One mL of the cocktail solution is recommended for the inhibition of protease activity found in 20 mL of cell lysate from 4g of E. coli cells.
Contains individual components, including AEBSF, EDTA, Bestatin, Pepstatin A, and E-64, each targeting specific types of proteases.
Lyophilized powder is stable for at least 2 years when stored unopened at -20°C.
Supplied with a vial of DMSO for preparation of a cocktail solution.
One mL of the cocktail solution is recommended for the inhibition of protease activity found in 20 mL of cell lysate from 4g of E. coli cells.
Attention
The lyophilized powder is stable for at least 2 years when stored unopened at -20°C. It is also supplied with a vial of DMSO. A prepared solution in DMSO and water will remain clear and colorless for approximately 24 hours at 4°C, before the inhibitors will precipitate out.
Notes préparatoires
A cocktail solution may be prepared by adding 1 mL of DMSO and 4 mL of deionized water to the 5 mL size or 5 mL of DMSO and 20 mL of deionized water to the 25 mL size. One mL of the cocktail solution is recommended for the inhibition of the protease activity found in 20 mL of cell lysate from 4g of E. coli cells.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Acute Tox. 4 Inhalation - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT RE 2 Inhalation
Organes cibles
Respiratory Tract
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
Classe de danger pour l'eau (WGK)
WGK 3
Équipement de protection individuelle
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Contenu apparenté
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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Does the protease inhibitor P8465 inhibit PreScission Protease? We utilized P8465 inhibitors in the purification of a GST-tagged protein fraction, and observed low activity of the PreScission protease (GE27-0843-01) on the dialyzed (using 10 kDa MWCO) GST-tagged protein fraction. Can you provide insight into the inhibition of the PreScission protease by this protease inhibitor cocktail?
1 answer-
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