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IMDM

With sodium bicarbonate and ʟ-glutamine, sterile liquid, suitable for cell culture

Synonyme(s) :

IMDM

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About This Item

Numéro MDL:
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

product name

Milieu de Dulbecco modifié par Iscove, With sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Composants

glucose: yes
phenol red: yes
NaHCO3: yes
L-glutamine: yes

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

Iscove′s Modified Dulbecco′s Medium contains selenium, additional amino acids and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate, and is a modification of Dulbecco′s Modified Eagle′s medium (DME). Iscove′s medium can be used to support murine B lymphocytes, bone marrow hemopoietic tissue, B cells stimulated with lipopolysaccharide, T lymphocytes, and various hybrid cells.

Application

Iscove′s Modified Dulbecco′s Medium has been used to culture:
  • human hepatocellular carcinoma (HCC) cell line HepG2 as monolayers
  • to culture synovial tissue samples
  • bacterial strain inoculum for stimulation of immune cell experiments

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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