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F2442

Sigma-Aldrich

Sérum de veau fœtal

Fetal Bovine Serum
1 of 1 reviewers received a sample product or took part in a promotion

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonyme(s) :

FBS, FCS, sérum, sérums

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About This Item

Numéro MDL:
MFCD00132239
Code UNSPSC :
12352207
eCl@ss :
32160409
Nomenclature NACRES :
NA.71
Source biologique:
bovine fetus
Origine:
USA origin
Stérilité:
sterile-filtered
Conditions d'expédition:
dry ice
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

bovine fetus

Niveau de qualité

500

Stérilité

sterile-filtered

Gamme de produits

FBS Classic

Composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

Origine

USA origin

Technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

Impuretés

≤10 EU/mL endotoxin

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Description générale

Le sérum de veau fœtal (SVF ou FBS en anglais) est un mélange complexe qui contient des molécules de bas et haut poids moléculaires et qui présente des fonctions optimales pour la stimulation et l'inhibition de la croissance. Le FBS contient des molécules comme des facteurs de croissance, des protéines, des vitamines, des oligoéléments, des hormones, etc. Ces molécules sont importantes pour la croissance et le maintien des cellules. C'est un supplément pour milieux de culture cellulaire d'usage courant.[1][2] Le FBS est obtenu à partir de sang de veau. Il est dépourvu de fibrine et de facteurs de coagulation. Son inactivation par la chaleur inhibe le système du complément.[3] Le FBS dérivé de sang coagulé est le supplément non défini le plus utilisé dans la culture des cellules eucaryotes et notamment des cellules de mammifères.[4][5] Il est généralement utilisé avec les milieux classiques mis au point dans les années 1950 et 1960. Ces derniers comprennent de nombreux milieux très prisés, comme le milieu essentiel minimum (MEM)[6], le milieu d'Eagle modifié par Dulbecco (DME ou DMEM), le milieu de Dulbecco modifié par Iscove (IMDM)[7], le milieu M199[8] et le milieu RPMI 1640 (Roswell Park Memorial Institute).[6]

Application

Le sérum de veau fœtal entre également dans la composition des milieux et liquides suivants :
  • milieu de croissance de cellules hybrides mélanocyte/macrophage périvasculaire (PVM/M)[9]
  • liquide tubaire de synthèse (SOF)[10]
  • milieu essentiel minimum d'Eagle (EMEM)[11]
  • milieu Neurobasal[12]
  • milieu 5A de Hyclone McCoy[13]
  • milieu de Hank[14]

Actions biochimiques/physiologiques

Le FBS reste un supplément pour milieux très prisé, car il assure de multiples fonctions en culture cellulaire. Il apporte des nutriments, des facteurs de croissance et d'adhésion et protège les cellules des dommages oxydatifs et de l'apoptose par des mécanismes qui sont difficiles à reproduire avec les milieux sans sérum.

Remarque sur l'analyse

  • A fait l'objet de tests pour rechercher la présence de bactéries, de virus et de mycoplasmes
  • Filtré trois fois sur membrane de 0,1 micron en conditions aseptiques

  • Taux d′endotoxines et d′hémoglobine testés

Autres remarques

Produit testé pour la présence de bactéries, de virus, de mycoplasmes et d'endotoxines et pour la teneur en hémoglobine. Produit filtré trois fois sur membrane de 0,1 µm en conditions aseptiques.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
19G057-A
24B128
0000321240
0000321240
23A316

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

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Fetal Bovine Serum

Pénicilline-streptomycine Solution stabilized, with 10,000 units penicillin and 10 mg streptomycin/mL, 0.1 μm filtered, BioReagent, suitable for cell culture

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P4333

Pénicilline-streptomycine

Axenic Procyclic Culture of L. tropica and L. donovani in Culture of FBS-free Medium
Mosa FM, et al.
Ife Journal of Science, 4B, 3088-3093 (2015)
MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member.
Grigoryev SA et al.
The Journal of Biological Chemistry, 274, 5626-5626 (1999)
Inhibiting tyrosine phosphorylation of protein kinase Cd (PKCd) protects the salivary gland from radiation damage.
Wie SM et al.
The Journal of Biological Chemistry, 289, 10900-10900 (2014)
Diana Lizeth Cervantes-Madrid et al.
Oncology letters, 13(3), 1411-1416 (2017-04-30)
The expression levels of cyclooxygenase (COX)-2 and the prostaglandin E2 (PGE2) content have been associated with poor prognosis in patients with colorectal cancer (CRC). There is a strong correlation between COX-2 expression and PGE2 production in tissues from CRC patients
Cunzhi Yu et al.
Frontiers in pharmacology, 8, 832-832 (2017-12-07)
Low solubility, tissue accumulation, and toxicity are chief obstacles to developing triptolide derivatives, so a better understanding of the pharmacokinetics and toxicity of triptolide derivatives will help with these limitations. To address this, we studied pharmacokinetics and toxicity of (5R)-5-hydroxytriptolide
Afficher tous les articles scientifiques apparentés

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Fluorescent Live Cell Imaging of LC3 Mediated Autophagy using LentiBrite™ Lentiviral Biosensors

High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Sérum de veau fœtal (FBS) – Ce que vous devez savoir

Révisez les bases de l'utilisation du sérum bovin (ou de veau) fœtal dans la culture de cellules, et redécouvrez comment la provenance et la réglementation affectent le prix des sérums impliqués dans les tests cellulaires.

Protocoles

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

Contenu apparenté

Evaluating of Stericup® Quick Release Filtration Devices for Fetal Bovine Serum (FBS) in Cell Culture

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Protocol Guide: Generation of Apical-Out Gastrointestinal Organoids

Step-by-step protocol for generating apical-out human gut organoids for microbiome, ADME/Tox, viral and gastrointestinal related disease research. See the complete organoid culture protocol.

Fetal Bovine Serum that Makes the Grade

Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.

Cell Culture Workflow

Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.

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Questions

1–10 of 15 Questions  

  1. How to heat inactivate this serum Fetal Bovine Serum USA origin,(Cat Log - F2442)

    1 answer

    1. Heat inactivation occurs when serum is heated to 56°C for a period of 30 minutes.

      Here's a protocol for heat inactivation:
      1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
      2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
      3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
      4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.

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  2. is this Heat Inactivate FBS?

    1 answer

    1. This product has not been heat inactivated.

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  3. can F2442 FBS be used for culturing peritoneal macrophages? what is the suitable one?

    1 answer

    1. This product is tested for suitability in mammalian cell culture. While it is not specifically tested on macrophages, it is subject to stringent specifications, including low endotoxin, and should perform well. Please see the link below to review a sample Certificate of Analysis:
      https://www.sigmaaldrich.com/coa/SIGMA/F2442/23A317

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  4. Can this f2442 fbs be used for culture expansion of mesenchymal stem cells? Please confirm Thanks

    1 answer

    1. Fetal Bovine Serum (Cat. no.: F2442) is suitable for use in mammalian cell culture. However, it is not tested specifically in mesenchymal stem cell culture. There is some evidence in the literature that this product would be suitable. See the link below for a relevant reference:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545441/

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  5. What is the difference between Fetal Bovine Serum F2442 and 12103C?

    1 answer

    1. Product No. 12103C meets most of the specifications of F2442.  Both products are from the same raw material suppliers and undergo the same stringent testing prior to release. However, the F2442 has been use-tested in hybridoma culture, whereas 12103C has not.

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  6. There is a precipitate in my serum.  Is my serum contaminated?

    1 answer

    1. Precipitates in serum can often appear as large round bodies, even as doublets per microscopic exam. This can be mistaken as a yeast contamination. Brownian motion may be mistaken for motility. These are lipid aggregates that will be readily dissolved when exposed to lipase. Also, these particles will not gram stain or show any growth in culture on aerobic, anaerobic, or fungal media and culture conditions. The performance of the material is not effected. Centrifugation or filtration of small volumes will remove these particles.

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  7. What is the cloning assay performed on Fetal Bovine Serum, Product F2442?

    1 answer

    1. The cloning efficiency assay is performed on each lot of FBS to verify that each lot will support clonal growth of myeloma cells and/or fusion products. The assay is performed using 5% and 10% concentrations of the FBS.

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  8. How is Fetal Bovine Serum, Product F2442, cell culture tested?

    1 answer

    1. Biological performance is assessed using Sigma cell culture medium supplemented with the serum and tested at a final concentration of 10%. Seeding efficiency, doubling time, and final cell density are measured on appropriate cell lines. Growth studies are carried through two subculture generations and cell growth is plotted as a logarithmic function of time in culture. During the testing period, cultures are examined microscopically for atypical morphology and evidence of cytotoxicity. Hybridoma -tested serum has been shown to support mouse myeloma cells and hybridoma cells in culture.

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  9. Is Fetal Bovine Serum, Product F2442, available as a charcoal stripped, de-lipidated serum?

    1 answer

    1. We offer Product No. F6765 (Fetal Bovine Serum, charcoal stripped). The charcoal stripping decreases the levels of hormones; it may also decrease the levels of lipids. If further reduction of lipid levels is required, you may need to treat the serum with fumed silica (e.g. Product No. S5130). Use 20 grams of S5130 per Liter of serum (not plasma). It is mixed for 3-4 hours, then centrifuged in 1 L containers for about 15 minutes at 2000 xg. The supernatant can then be decanted, and filtered if desired. NOTE: Silica is extremely fluffy, and care must be taken not to inhale its dust. Allow for about 15% loss of the serum due to adsorption.

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  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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Reviews

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    최고의 갓성비 FBS!

    정기배송으로 진행시 가격도 50만원으로 저렴하고 정해진 날짜에 와서 좋아요!
    DMSO같이 많이 쓰는 제품도 media와 같이 묶어서 사용하면 더 저렴해지더라구요 ㅎ

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