This serum product is collected from USDA approved abbatoirs and pooled for production. Analytical specifications for Endotoxin, Total Protein, Osmolality, and pH are assigned, and these values reflect a high level of consistency. The maximum allowable hemoglobin content is 25 mg/dL. This value, as well as other measured components, may vary significantly from lot to lot. Note that samples of available lots may be requested for screening purposes. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/f0926#product-documentation
F0926
Sérum de veau fœtal
Fetal Bovine Serum
USDA approved, sterile-filtered, suitable for cell culture
Synonyme(s) :
FBS, FCS, sérum, sérums
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About This Item
Source biologique:
bovine fetus
Stérilité:
sterile-filtered
Conditions d'expédition:
dry ice
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Source biologique
bovine fetus
Niveau de qualité
Stérilité
sterile-filtered
Gamme de produits
FBS Classic
Qualité
USDA approved
Composition
Hemoglobin, ≤25 mg/dL
Technique(s)
cell culture | mammalian: suitable
Impuretés
≤10 EU/mL endotoxin
Conditions d'expédition
dry ice
Température de stockage
−20°C
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Description générale
Fetal bovine serum (FBS) or fetal calf serum is the blood fraction remaining after the natural coagulation of blood. It comes from the blood drawn from a bovine fetus via a closed system of collection at the slaughterhouse.
Application
Le sérum de veau fœtal originaire de pays autres que les États-Unis et approuvé par l'USDA est utilisé dans une large gamme d'applications de culture cellulaire. Il apporte aux cellules en culture de nombreux facteurs d'amélioration de la survie et de promotion de la croissance non définis.
Le sérum de veau fœtal a été utilisé pour les cultures cellulaires suivantes :
Le sérum de veau fœtal a été utilisé pour les cultures cellulaires suivantes :
- culture primaire de macrophages humains (les cellules mononucléaires ont été remises en suspension dans du milieu RPMI (Roswell Park Memorial Institute) contenant du sérum de veau fœtal (FBS) ainsi que d'autres composants).[1]
- culture tissulaire (les MEF ont été mis en culture dans du DMEM enrichi de FBS et d'autres composants).[2]
- isolement et culture 3D d'organoïdes épithéliaux mammaires primaires[3]
- culture cellulaire et milieu (les HUVEC ont été cultivées dans un milieu de croissance de cellules endothéliales-2 (ECGM-2) enrichi de FBS et de facteurs de croissance)[4]
Adéquation
USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.
Remarque sur l'analyse
- A fait l'objet de tests pour rechercher la présence de bactéries, de virus et de mycoplasmes
- Filtré trois fois sur membrane de 0,1 micron en conditions aseptiques
- Taux d′endotoxines et d′hémoglobine testés
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves
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Articles
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.
Révisez les bases de l'utilisation du sérum bovin (ou de veau) fœtal dans la culture de cellules, et redécouvrez comment la provenance et la réglementation affectent le prix des sérums impliqués dans les tests cellulaires.
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
Protocoles
This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.
Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.
Contenu apparenté
Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.
Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.
Découvrez notre gamme de sérums de veau fœtal (FBS) et identifiez le FBS adapté à vos besoins et à votre budget. Demander un échantillon de sérum.
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What is the lot-to-lot consistency of this product?
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Is this item heat inactivated?
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This product is not heat inactivated.
Please view product F4135 for a heat-inactivated Fetal Bovine Serum:
https://www.sigmaaldrich.com/product/sigma/f4135.Helpful?
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Does Sigma-Aldrich offer fetal bovine serum (FBS) that is antibiotic-free?
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FBS can be tested for the presence of antibiotics. Products F2442, F0926, and F6178, Fetal Bovine Serum are routinely tested for Tetracycline (by far the most commonly used antibiotic in animals) and the results are reported on the lot specific Certificate of Analysis.Per the USDA, Antibiotics may be given to prevent or treat disease in cattle. A "withdrawal" period is required from the time antibiotics are administered until it is legal to slaughter the animal. This is so residues can exit the animal's system. FSIS randomly samples cattle at slaughter and tests for residues. Data from this Monitoring Plan have shown a very low percentage of residue violations.The USDA does random sampling of all cattle submitted for slaughter in USDA inspected facilities for antibiotics. The mandatory withdrawal period is 24 hours. Based on withdrawal periods required by the USDA, animal testing at slaughter and finished product testing, our serum is antibiotic free.
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Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?
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Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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