85578

Spermidine trihydrochloride

BioXtra, ≥99.5% (AT)

• Synonyme(s) : N-(3-Aminopropyl)-1,4-butanediamine trihydrochloride
• Formule linéaire : NH₂(CH₂)₃NH(CH₂)₄NH₂ · 3HCl
• Numéro CAS: 334-50-9
• Poids moléculaire : 254.63
• Beilstein: 3552356
• Numéro CE : 206-379-0
• Numéro MDL: MFCD00012918
• Code UNSPSC : 12352116
• ID de substance PubChem : 57653005
• Nomenclature NACRES : NA.26

Propriétés

• Gamme de produits: BioXtra
• Niveau de qualité: 100
• Essai: ≥99.0% (TLC); ≥99.5% (AT)
• Forme: powder
• Impuretés: insoluble matter, passes filter test
• pH: 3.0-6.0 (25 °C, 1 M in H₂O)
• Pf: 257-259 °C (lit.)
• Solubilité: H₂O: 1 M at 20 °C, clear, colorless
• Traces d'anions: sulfate (SO₄^2-): ≤50 mg/kg
• Traces de cations: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤10 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 1 M in H₂O
• Absorption UV: λ: 260 nm A_max: 0.2; λ: 280 nm A_max: 0.1
• Chaîne SMILES: Cl[H].Cl[H].Cl[H].NCCCCNCCCN
• InChI: 1S/C7H19N3.3ClH/c8-4-1-2-6-10-7-3-5-9;;;/h10H,1-9H2;3*1H
• Clé InChI: LCNBIHVSOPXFMR-UHFFFAOYSA-N

Description

Description générale

Spermidine trihydrochloride is an aliphatic polyamine. Its crystalline structure has been studied.[1] It has been reported to preserve the nuclei and prevent proteolytic degradation of histones during the macronuclei isolation from Tetrahymena pyriformis.[2]

Application

Spermidine trihydrochloride is used for the following applications:

• DNA extraction and restriction endonuclease digestion[3]
• Nuclear transplantation[4]
• During the chemical analyses of the squid species Todaropsis eblanae[5]
• As one of the agents in intercalating drug.[6]
• Platelet aggregation[7]

It may be used in the synthesis of palladium complexes like [sperH₃]₂[PdCl₄]₃, [PdCl₂(sperH)]₂[PdCl₄], and [(PdCl₂)₃(sper)₂] by reacting with K₂PdCl₄ and PdCl₂.[8]

Actions biochimiques/physiologiques

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; Stimulates T₄ polynucleotide kinase activity.

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T₄ polynucleotide kinase activity.

Attention

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

Autres remarques

Buffer component for chromosome isolation in bivariate flow cytogenetic analysis and sorting[9].

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H315,H319
• Conseils de prudence: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Classification des risques: Eye Irrit. 2 - Skin Irrit. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves