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49940

Millipore

ONPG Disks

suitable for microbiology

Synonyme(s) :

β-Galactosidase Test Disks, 2-Nitrophenyl-β-D-galactopyranoside Disks

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About This Item

Code UNSPSC :
41171621
Nomenclature NACRES :
NA.85

Agence

according to GB 4789.4-2016

Gamme de produits

BioChemika

Durée de conservation

limited shelf life, expiry date on the label

Technique(s)

microbe id | specific enzyme detection: suitable

Application(s)

environmental
food and beverages
microbiology

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Adéquation

Escherichia coli
bacteria

Description générale

ONPG Disks are used to detect the presence of β-galactosidase, an enzyme found in lactose-fermenting organisms. Lactose utilization depends upon two enzymes: β-galactoside permease, which catalyzes transport of lactose into the cell, and β-galactosidase, which breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides including the ONPG (o-nitrophenyl-β-D-galactopyranose) substrate. ONPG hydrolysis results in the release of galactose, and the yellow chromogenic compound, o-nitrophenol. The test substrate, ONPG, does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period even for late lactose fermenters.

Conditionnement

Sterile filter paper discs (50 pcs, diameter 6 mm) impregnated with o-nitrophenyl-β-D-galactopyranose

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
BCCL6201
BCCM1925
BCCK3268
BCCJ4470
BCCG9930

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Leann Clark et al.
Microbiology (Reading, England), 157(Pt 7), 2072-2083 (2011-04-16)
Most studies on Salmonella enterica serovar Typhimurium infection focus on strains ATCC SL1344 or NTCC 12023 (ATCC 14028). We have compared the abilities of these strains to induce membrane ruffles and invade epithelial cells. S. Typhimurium strain 12023 is less

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