11699121001

PCR Buffer Set

suitable for PCR, pkg of 2 × 2 mL

• Code UNSPSC : 41116133

Propriétés

• Conditionnement: pkg of 2 × 2 mL
• Fabricant/nom de marque: Roche
• Technique(s): PCR: suitable
• Température de stockage: −20°C

Description

Description générale

The kit-provided PCR buffer without MgCl₂, in combination with the kit-provided MgCl₂ stock solution, are used for the individual adjustment of the Mg²⁺ concentration. In most PCR applications, a concentration of 1.5 mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200 μM for each nucleotide. To optimize the PCR, titrate to determine the optimal Mg²⁺ concentration for high PCR specificity and amplicon yield.

Application

For optimization of the magnesium-chloride concentration in the polymerase chain reaction (PCR) using Taq DNA Polymerase.

Conditionnement

1 set containing 2 components

1 set containing 3 components

Qualité

Function test: Each lot of buffer is tested in PCR.
Absence of contaminants: No exo- or endonuclease activity is detectable according to the current Quality Control procedures.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Composants de kit seuls

• MgCl₂ Stock Solution 25 mM
• PCR Buffer without Mg²⁺ 10x concentrated
• Alkaline Phosphatase Substrate, CSPD
• Enhancer, Emerald II
• Assay Buffer

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash