Accéder au contenu
MilliporeSigma
Toutes les photos(2)

Documents

14533

Sigma-Aldrich

bisBenzimide H 33342 trihydrochloride

for fluorescence, ≥97.0% (HPLC)

Synonyme(s) :

2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Formule empirique (notation de Hill):
C27H28N6O · 3HCl · xH2O
Numéro CAS:
875756-97-1
Poids moléculaire :
561.93 (anhydrous basis)
Numéro Beilstein :
1234011
Numéro CE :
245-691-1
Numéro MDL:
MFCD00012678
Code UNSPSC :
12161505
ID de substance PubChem :
57647173
Nomenclature NACRES :
NA.25

Qualité

for fluorescence

Pureté

≥97.0% (HPLC)

Fluorescence

λex 340 nm; λem 510 nm
λex 355 nm; λem 465 nm in TE buffer; DNA

Température de stockage

2-8°C

Chaîne SMILES 

Cl[H].Cl[H].Cl[H].CCOc1ccc(cc1)C2=NCc3cc(ccc3N2)C4=NCc5cc(ccc5N4)N6CCN(C)CC6

InChI

1S/C29H32N6O.3ClH/c1-3-36-25-8-4-20(5-9-25)28-30-18-22-16-21(6-10-26(22)32-28)29-31-19-23-17-24(7-11-27(23)33-29)35-14-12-34(2)13-15-35;;;/h4-11,16-17H,3,12-15,18-19H2,1-2H3,(H,30,32)(H,31,33);3*1H

Clé InChI

FYEVKHPLBHLWHK-UHFFFAOYSA-N

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Application

bisBenzimide H 33342 trihydrochloride can be used to study biochemicals and reagents, fluorescent probes, labels, particles and stains, luminescent compounds and detection and wavelength index. bisBenzimide H 33342 trihydrochloride, Hoechst 33342, has been used in a study to investigate the effects of inorganic and organic arsenic compounds on human T-lymphoblastoid leukemia cells. Hoechst 33342 has also been used in a study to examine the effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP.
Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

Actions biochimiques/physiologiques

Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.

Conditionnement

Bottomless glass bottle. Contents are inside inserted fused cone.

Autres remarques

Suitable for vital DNA staining of a variety of cell types; As a probe of membrane permeability in mammalian cells

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

08168

Timestrip Plus™ 8 °C

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 6

1 of 6

HOECHST 2495 AldrichCPR

R158569

HOECHST 2495

Pyronin Y for microscopy (Bot., Fl., Hist.)

Sigma-Aldrich

83200

Pyronin Y

Iodure de propidium ≥94% (HPLC)

Sigma-Aldrich

81845

Iodure de propidium

Pyronin Y certified by the Biological Stain Commission, Dye content 50 %

Sigma-Aldrich

213519

Pyronin Y

DAPI for nucleic acid staining

Sigma-Aldrich

D9542

DAPI

Millipore

Millipore

WBAVDFL01

Immobilon® Block - FL (bloqueur fluorescence)

W M Grogan et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 29(6), 738-746 (1981-06-01)
The dye Hoechst 33342 and a 2-parameter cell sorter have been used to measure DNA content in viable testis cells and to sort pachytene spermatocytes and round spermatids from adult mouse testis to virtually 100% homogeneity. Early diploid spermatogenic cells
M J Lydon et al.
Journal of cellular physiology, 102(2), 175-181 (1980-02-01)
A procedure has been investigated for sorting viable cells according to their DNA content. Cells are stained with the U.V. activated fluorochromes 4'6-diamidino-2-phenylindole (DAPI), Hoechst 33258 or Hoechst 33342, and sorted with a Fluorescence Activated Cell Sorter. Hoechst 33342 is
M E Lalande et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(1), 363-367 (1981-01-01)
Flow cytometric analysis of the uptake of the DNA-specific and fluorescent probe Hoechst 33342 (HO342) offers a simple and rapid method for measuring membrane transport rates in mammalian cells and identifying cellular subpopulations that differ in their membrane transport rates.
Steven S Welc et al.
Nature communications, 10(1), 2788-2788 (2019-06-28)
Many potentially therapeutic molecules have been identified for treating Duchenne muscular dystrophy. However, targeting those molecules only to sites of active pathology is an obstacle to their clinical use. Because dystrophic muscles become extensively inflamed, we tested whether expressing a therapeutic
Ganna Aleshcheva et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 29(6), 2303-2314 (2015-02-15)
Real and simulated microgravity induce a variety of changes in human cells. Most importantly, changes in the cytoskeleton have been noted, and studies on microtubules have shown that they are gravisensitive. This study focuses on the effects of short-term real
Afficher tous les articles scientifiques apparentés

Contenu apparenté

DNA Detection

Nancy-520 for DNA Detection and Quantitation

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique