14533

bisBenzimide H 33342 trihydrochloride

suitable for fluorescence, ≥97.0% (HPLC)

• Synonyme(s) : 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide
• Formule empirique (notation de Hill) : C₂₇H₂₈N₆O · 3HCl · xH₂O
• Numéro CAS: 875756-97-1
• Poids moléculaire : 561.93 (anhydrous basis)
• UNSPSC Code: 12161505
• NACRES: NA.25
• PubChem Substance ID: 57647173
• EC Number: 245-691-1
• Beilstein/REAXYS Number: 1234011
• MDL number: MFCD00012678

Propriétés

• Quality Segment: 200
• assay: ≥97.0% (HPLC)
• fluorescence: λ_ex 340 nm; λ_em 510 nm, λ_ex 355 nm; λ_em 465 nm in TE buffer; DNA
• suitability: suitable for fluorescence
• storage temp.: 2-8°C
• SMILES string: Cl[H].Cl[H].Cl[H].CCOc1ccc(cc1)C2=NCc3cc(ccc3N2)C4=NCc5cc(ccc5N4)N6CCN(C)CC6
• InChI: 1S/C29H32N6O.3ClH/c1-3-36-25-8-4-20(5-9-25)28-30-18-22-16-21(6-10-26(22)32-28)29-31-19-23-17-24(7-11-27(23)33-29)35-14-12-34(2)13-15-35;;;/h4-11,16-17H,3,12-15,18-19H2,1-2H3,(H,30,32)(H,31,33);3*1H
• InChI key: FYEVKHPLBHLWHK-UHFFFAOYSA-N

Description

Application

Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

bisBenzimide H 33342 trihydrochloride can be used to study biochemicals and reagents, fluorescent probes, labels, particles and stains, luminescent compounds and detection and wavelength index. bisBenzimide H 33342 trihydrochloride, Hoechst 33342, has been used in a study to investigate the effects of inorganic and organic arsenic compounds on human T-lymphoblastoid leukemia cells. Hoechst 33342 has also been used in a study to examine the effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP.

Biochem/physiol Actions

Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Suitable for vital DNA staining of a variety of cell types; As a probe of membrane permeability in mammalian cells

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves