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MAB350

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Anti-Enkephalin Antibody, clone NOC1

culture supernatant, clone NOC1, Chemicon®

Synonyme(s) :

Anti-CBF, Anti-CBF2, Anti-HSP-CBF, Anti-NOC1

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

culture supernatant

Type de produit anticorps

primary antibodies

Clone

NOC1, monoclonal

Espèces réactives

guinea pig, rabbit, rat, hamster

Fabricant/nom de marque

Chemicon®

Technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable

Isotype

IgG

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CEBPZ(10153)

Spécificité

Met5- and Leu5-enkephalin. Displays about 40% cross-reactivity with C-terminal extended Met-enkephalin hexapeptides and 7% cross-reactivity with the extended heptapeptide (-Arg-Phe-OH) but does not recognize pro-enkephalin or other endogenous peptides (Cuello, 1984). By immunohistochemistry the antibody lables all sites that are known to exppress enkephalin and shows no cross-reactivity to beta-endorphin or dynorphin (Cuello, 1984; MacMillan and Cuello, 1986).

Immunogène

Leu5-enkephalin conjugated to BSA.

Application

Detect Enkephalin using this Anti-Enkephalin Antibody, clone NOC1 validated for use in IC, IH.
Immunohistochemistry: 1:100-1:400 on 10 μm cryostat sections of rat and guinea-pig brain stem using fluorescently labled secondary antibodies for detection (Cuello, 1984; MacMillan and Cuello, 1986).

Immunocytochemistry: 1:300 on primary cultures of rat striatal and hippocampal neurons using a Cy2 or Cy3 conjugated secondary antibody for detection (Saudou, 1998).

Radioimmunoassay (Cuello, 1984)

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neuroinflammation & Pain

Hormones & Receptors

Forme physique

Lyophilized Supernatant, preservative free.

Stockage et stabilité

Lyophilized antibody can be kept at 2-8°C for up to 3 months and should be kept at -20°C for long term storage. Reconstitute with 100μL sterile DI water. Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogrammes

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Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

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Chen, P; Lin, D; Giesler, J; Li, C
The Journal of Comparative Neurology null
Kristen N Segovia et al.
The European journal of neuroscience, 35(8), 1354-1367 (2012-04-03)
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