Global chromatin relabeling accompanies spatial inversion of chromatin in rod photoreceptors.

Global chromatin relabeling accompanies spatial inversion of chromatin in rod photoreceptors.

Science advances (2021-09-25)

Cheryl L Smith, Yemin Lan, Rajan Jain, Jonathan A Epstein, Andrey Poleshko

PMID34559565

RESUMO

[Figure: see text].

MATERIAIS

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D9542

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DAPI, for nucleic acid staining

A4503

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Albumina sérica bovina, cold ethanol fraction, pH 5.2, ≥96%

SML1046

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CHIR99021, ≥98% (HPLC)

G2500

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Gelatina, gel strength 300, Type A

S2002

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Sodium azide, ReagentPlus^®, ≥99.5%

ESG1106

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Proteína LIF recombinante de camundongo ESGRO^®, ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^6 units/ml

06-599

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Anticorpo antiacetil-histona H3, from rabbit

PZ0162

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PD 0325901, ≥98% (HPLC)

02370

Sigma-Aldrich

Propil galato, for microscopy, ≥98.0% (HPLC)

06-866

Sigma-Aldrich

Anticorpo antiacetil-histona H4, serum, Upstate^®