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Discovery® C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 10 mm, HPLC Column

Sinônimo(s):

Discovery RP18 HPLC Column

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About This Item

Código UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Coluna de HPLC C18 Discovery®, 5 μm particle size, L × I.D. 25 cm × 10 mm

Materiais

stainless steel column

Agency

suitable for USP L1

linha de produto

Discovery®

Características

endcapped

fabricante/nome comercial

Discovery®

embalagem

1 ea of

Extensão da rotulagem

12% Carbon loading

Parâmetros

≤70 °C temp. range
400 bar pressure (5801 psi)

técnica(s)

HPLC: suitable
LC/MS: suitable

C × D.I.

25 cm × 10 mm

área da superfície

300 m2/g

cobertura de superfície

3 μmol/m2

Impurezas

<10 ppm metals

matriz

silica gel, high purity, spherical particle platform
fully porous particle

Grupo ativo da matriz

C18 (octadecyl) phase

tamanho de partícula

5 μm

tamanho de poro

180 Å

operating pH range

2-8

aplicação(ões)

food and beverages

técnica de separação

reversed phase

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Categorias relacionadas

Descrição geral

Use a coluna C18 Discovery® para qualquer método que especifique C18. A forma de pico, reprodutibilidade e estabilidade excepcionais significam que essa é a coluna ideal para todos os métodos de C18, desde os mais complicados até os de rotina.

Aplicação


  • Development and Validation of a Reversed-Phase HPLC Method with UV Detection for the Determination of L-Dopa in Vicia faba L. Broad Beans.: Highlights the application of the Discovery® C18 HPLC Column in developing a method for the accurate quantification of L-Dopa in natural sources, which is essential for studies in neuropharmacology and natural product research (Tesoro et al., 2022).

  • Simultaneous estimation of paclitaxel and erlotinib in plasma by liquid chromatography/(+) electrospray tandem mass spectrometry: application in formulation development and pharmacokinetics.: This study employs the Discovery® C18 HPLC Column to enhance the simultaneous analysis of paclitaxel and erlotinib, critical for cancer therapy research and formulation development (Khuroo et al., 2022).

Características e benefícios

  • Excelente reprodutibilidade
  • Forma de pico excepcional para analitos básicos e ácidos
  • Separações por LC-MS estáveis, com pouco vazamento
  • Separação de peptídeos e proteínas pequenas
  • Menor hidrofobicidade que muitas colunas C18 comparáveis, o que propicia uma análise mais rápida

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Informações legais

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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T R Shantha Kumar et al.
Journal of pharmaceutical and biomedical analysis, 38(1), 173-179 (2005-05-24)
A simple, precise and accurate isocratic reverse-phase liquid chromatography method with programmed wavelength detection has been validated to quantify DRF-4367 and Phenol red, simultaneously for application in rat in situ single pass intestinal perfusion study to assess intestinal permeability of
Abbas Khan et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(24), 2423-2429 (2011-07-26)
A novel isocratic reversed-phase high performance liquid-chromatography/ultraviolet detection method for simultaneous determination of cefdinir and cefixime in human plasma was developed and validated after optimization of various chromatographic conditions and other experimental parameters. Sample preparation based on a simple extraction
Himal Paudel Chhetri et al.
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 22(5), 483-487 (2014-12-05)
This study presents the optimization of a simple HPLC-UV method for the determination of metformin in human plasma. Ion pair separation followed by UV detection was performed on deproteinized human plasma samples. The separation was carried out on a Discovery
Ramisetti Nageswara Rao et al.
Journal of separation science, 32(9), 1312-1322 (2009-04-29)
Modafinil, adrafinil and their related substances were synthesized and analyzed by RP-LC with ESI-MS/MS. The ionization mode, polarity, cone voltage, and chromatographic conditions were evaluated. The optimum LC-MS conditions to obtain fragment ions indispensable for identification of the structures were
T Nagano et al.
Journal of dental research, 88(9), 823-828 (2009-09-22)
Mmp-20 and Klk4 are the two key enamel proteases. Can both enzymes process amelogenin to generate the major cleavage products that accumulate during the secretory stage of amelogenesis? We isolated Mmp-20 and Klk4 from developing pig teeth and used them

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