The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Outras notas
Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
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