WTA1
TransPlex® Whole Transcriptome Amplification Kit
DNA polymerase separate.
Sinônimo(s):
Transcriptome Amplification Kit
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About This Item
Código UNSPSC:
41121800
NACRES:
NA.55
Produtos recomendados
técnica(s)
whole genome amplification: suitable
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Descrição geral
TransPlex®, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3′-bias. Microgram quantities of amplification product generated from tissue, cultured cells, formalin-fixed samples, or serum are suitable for downstream applications such as qPCR and microarray analyses.
Aplicação
TransPlex® Whole Transcriptome Amplification Kit has been used to synthesize double-stranded cDNA. It has also been used in the amplification of cDNA.
Suitable for use with downstream applications including:
- qPCR
- microarray analysis
- cloning
Características e benefícios
- Amplification of total RNA in less than 4 hours with less than 30 minutes of "hands-on" time
- Only 5 ng of starting material required to produce a highly representative library from total RNA
- Microgram quantities of amplification product generated from intact RNA from tissue, cultured cells, serum, or degraded RNA from formalin-fixed paraffin-embedded samples
Princípio
The WTA process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. As polymerization proceeds, displaced single strands serve as new templates for primer annealing and extension. The resultant OmniPlex cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence, is then amplified by PCR with the universal primer to produce WTA product.
Informações legais
TransPlex is a registered trademark of Rubicon Genomics, Inc.
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Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2
Código de classe de armazenamento
10 - Combustible liquids
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Protocolos
TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.
Conteúdo relacionado
Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications
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