T1819

Trizma^® hydrochloride solution

pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

• Sinônimo(s): Tris hydrochloride solution
• Código UNSPSC: 12161700
• NACRES: NA.25

Propriedades

• grau: BioPerformance Certified; for molecular biology
• Nível de qualidade: 200
• Formulário: liquid
• concentração: 1 M
• técnica(s): cell culture | mammalian: suitable
• Impurezas: DNase, RNase, Protease, none detected; bioburden, tested; endotoxin, tested; ≤5 ppm Heavy metals (as Pb)
• pH: 7.0
• faixa de pH útil: 7.0-9.0
• Absorção: ≤0.05 at 290 at 40%

Descrição

Descrição geral

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Tris(hydroxymethyl)aminomethane (Tris) is a standard buffer in biological sciences and has a pH buffering range between 7.0 - 9.0.[1] It is applicable in electrophoretic studies and capillary electrochromatography.[2]

Aplicação

Trizma^® hydrochloride solution has been used:

• as a buffer in reverse transcription and DNase I treatment reactions for RNA isolated from Toxoplasma gondii[3]
• in the preparation of tris- ethylenediaminetetraacetic acid (EDTA) (TE) buffer for bacterial pellet resuspension for plasmid isolation studies[4]
• for polylactic acid nanoparticle resuspension, storage, and pH-based stability studies[5]

Informações legais

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type ABEK (EN14387) respirator filter