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SHC016V

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles

Targets no known genes from any species

Sinônimo(s):

MISSION®, MISSION® Control Transduction Particles, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control

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About This Item

Código UNSPSC:
41106609
NACRES:
NA.51
Preço e disponibilidade não estão disponíveis no momento.

Nível de qualidade

100

linha de produto

MISSION®

concentração

≥1x106 VP/ml (via p24 assay)

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

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Descrição geral

The MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION® shRNA library clones.[1][2] This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Transduction Particles are provided as 200 μL at 1 x 106 TU/mL via p24 assay.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Aplicação

MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles have been used to generate 3T3-L1 (pre-adipocytes) control cell lines.[3]

Nota de análise

To see more application data, protocols, vector maps visit sigma.com/shrna.

Informações legais

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
01212208MN
03162006MN
07181827MN

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