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SHC004V

Sigma-Aldrich

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Transduction Particles

shRNA sequence targeting tGFP

Sinônimo(s):

MISSION®, MISSION® Control Transduction Particles

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About This Item

Código UNSPSC:
41106609
NACRES:
NA.51

linha de produto

MISSION®

concentração

≥1x106 VP/ml (via p24 assay)

técnica(s)

capture ELISA: 106 TU/mL using p24

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

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Descrição geral

The MISSION TurboGFP shRNA Control Transduction Particles contain an shRNA sequence targeting TurboGFP. The TurboGFP shRNA Control Particles are useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
The TurboGFP shRNA control transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-TurboGFP shRNA (SHC004). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Aplicação

To see more application data, protocols, vector maps visit sigma.com/shrna.

Informações legais

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.

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Descrição
Preços

SHC002

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes

SHC001V

MISSION® pLKO.1-puro Empty Vector Control Transduction Particles, Contains no shRNA insert

SHC003

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency

SHC002V

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, Targets no known mammalian genes

SHC003V

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles, Green fluorescent protein marker to monitor transduction efficiency

SHC004

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA, shRNA sequence targeting tGFP

SHM01

MISSION® ExpressMag® Super Magnetic Kit, Increases transduction efficiency

SHM02

MISSION® ExpressMag® 96-Well Magnetic Kit, Increases transduction efficiency

SHC016V

MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles, Targets no known genes from any species

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Mariano J Alvarez et al.
Nature genetics, 50(7), 979-989 (2018-06-20)
We introduce and validate a new precision oncology framework for the systematic prioritization of drugs targeting mechanistic tumor dependencies in individual patients. Compounds are prioritized on the basis of their ability to invert the concerted activity of master regulator proteins

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