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S7897

Sigma-Aldrich

Sarcosine Oxidase from Bacillus sp.

lyophilized powder, 25-50 units/mg solid

Sinônimo(s):

Sarcosine: oxygen oxidoreductase (demethylating)

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1000 UNITS
R$ 5.166,00

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Previsão de entrega em12 de abril de 2025

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About This Item

Número CAS:
9029-22-5
Número da licença da enzima:
1.5.3.1 (BRENDA, IUBMB)
Número CE:
232-850-5
Número MDL:
MFCD00132221
Código UNSPSC:
12352204
NACRES:
NA.54

R$ 5.166,00

Previsão de entrega em12 de abril de 2025

Formulário

lyophilized powder

Nível de qualidade

200

atividade específica

25-50 units/mg solid

peso molecular

44 kDa

temperatura de armazenamento

−20°C

Descrição geral

Monomeric sarcosine oxidase (MSOX) is a flavoenzyme that catalyzes the oxidative demethylation of sarcosine (N-methylglycine) to yield glycine, formaldehyde, and hydrogen peroxide. Monomeric sarcosine oxidase can oxidize other secondary amino acids such as N-methyl-L-alanine, N-ethylglycine, and L-proline. [1]
Sarcosine oxidase is a 44 kDa protein with covalently bound 1 mol of flavin adenine dinucleotide (FAD). It belongs to the family of amino acid oxidases with bound FAD.[2]

Aplicação

Sarcosine oxidase from Bacillus sp. has been used as a positive control for detection of proteins with covalently bound flavin adenine dinucleotide (FAD) by UV.[3] It has also been used in co-immobilization with other enzymes on platinum electrode for preparation of creatinine biosensor.[4]
Sarcosine oxidase has been used in a study as part of a multienzyme cascade, that when immobilized constructed amperometric biosensors. [5] Sarcosine oxidase has also been used in a study to investigate oxidation of amines by flavoproteins. [6]

Definição da unidade

One unit will form 1.0 μmole of formaldehyde from sarcosine per min at pH 8.3 at 37 °C.

forma física

No stabilizers added

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
0000281578
0000266496
0000258361
0000266496
0000258361

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Optimization of the production of Chondrus crispus hexose oxidase in Pichia pastoris
Wolff AM, et al.
Protein Expression and Purification, 22(2), 189-199 (2001)
Paul F Fitzpatrick
Archives of biochemistry and biophysics, 493(1), 13-25 (2009-08-05)
Many flavoproteins catalyze the oxidation of primary and secondary amines, with the transfer of a hydride equivalent from a carbon-nitrogen bond to the flavin cofactor. Most of these amine oxidases can be classified into two structural families, the D-amino acid
Rastislav Monošík et al.
Analytical biochemistry, 421(1), 256-261 (2011-11-10)
Amperometric biosensors based on gold planar or nanocomposite electrode containing multiwalled carbon nanotubes for determination of glycerol were developed. The biosensors were constructed by immobilization of a novel multienzyme cascade consisting of glycerol kinase/creatine kinase/creatinase/sarcosine oxidase/peroxidase between a chitosan "sandwich."
Handbook of Flavoproteins: Oxidases, Dehydrogenases and Related Systems, 196-196 (2012)
Kangping Guo et al.
Applied microbiology and biotechnology, 73(3), 559-566 (2006-09-16)
A heat-stable sarcosine oxidase produced by Bacillus sp. BSD-8 (SOX) had been studied and its complete gene sequence, which contained 1,164 bp nucleotides and encoded a protein of 387 amino acids, was obtained by DNA Walking method. The sox gene
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