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S6147

Sigma-Aldrich

Sephadex® G-100

BioReagent, DNA grade, for molecular biology, medium

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R$ 2.271,00
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About This Item

Número CAS:
9050-94-6
Número MDL:
MFCD00132235
Código UNSPSC:
23151817
NACRES:
NA.52

R$ 2.271,00

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grau

Molecular Biology
for molecular biology

linha de produto

BioReagent

Formulário

beads

técnica(s)

buffer exchange: suitable

Grupo ativo da matriz

phase

inchaço

1 g swells to 9-11 mL

diâmetro da esfera

40-120 μm

tamanho de poro

exclusion limit (25 bp dsDNA; (rA)27)

pH

2-10

aplicação(ões)

life science and biopharma

compatibilidade

Cytiva

atividade externa

DNase, RNase, none detected

temperatura de armazenamento

room temp

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Descrição geral

Sephadex® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large molecules. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Aplicação

Sephadex® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.[1]

Características e benefícios

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Informações legais

Sephadex is a registered trademark of Cytiva

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
0000353761
0000353762
0000353762
0000353761
0000324568

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Isolation, purification and physicochemical characterization of polyphenoloxidases (PPO) from a dwarf variety of banana (Musa cavendishii, L)
Galeazzi MAM et al
Journal of Food Science, 46, 150-155 (1981)
Evan S Leibner et al.
Acta biomaterialia, 5(5), 1389-1398 (2009-01-13)
Analytical protocol greatly influences the measurement of human serum albumin (HSA) adsorption to commercial expanded polytetrafluororethylene (ePTFE) exhibiting superhydrophobic wetting properties. Degassing of buffer solutions and evacuation of ePTFE adsorbent to remove trapped air immediately prior to contact with protein
Zhipeng Li et al.
International immunopharmacology, 14(2), 145-149 (2012-07-10)
The study is to investigate the effect of compound IMMLG5521, a coumarin derivative, on lung inflammation induced by Sephadex in rats. Sephadex led to massive granulomas, infiltration of neutrophils and eosinophils, the increase of TNF-α level in BALF and lung
Dae-Young Lee et al.
Journal of medicinal food, 15(4), 399-405 (2012-02-10)
Grains of sugary rice were extracted with 80% aqueous methanol, and the concentrated extracts were successively partitioned using ethyl acetate, n-butanol, and water. From the n-butanol fractions, four flavonoid glycosides were isolated through repeated silica gel, octadecyl silica gel, and
Lila Boulekbache-Makhlouf et al.
Phytochemical analysis : PCA, 24(2), 162-170 (2012-08-30)
Eucalyptus species are widely cultivated in Mediterranean regions. Moreover, plants of this family have been utilized for medicinal purposes. A number of studies have been devoted to the identification of eucalypt phenolics, all of them have focused on specific families
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