R7647
Random Nonamers
for use as primers in cDNA synthesis, 50 μM in H2O
Sinônimo(s):
Oligodeoxynucleotides
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100 μL
R$ 629,00
R$ 629,00
Disponível para enviar em01 de abril de 2025Detalhes
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100 μL
R$ 629,00
About This Item
Código UNSPSC:
41105500
NACRES:
NA.31
R$ 629,00
Disponível para enviar em01 de abril de 2025Detalhes
Produtos recomendados
grau
Molecular Biology
for molecular biology
Formulário
liquid
uso
0.1 mL sufficient for 100 RT-PCR reactions
concentração
50 μM in H2O
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
Descrição geral
Random Nonamers are random sequences of nine deoxyribonucleotides (9-mers) used to prime mRNA, with or without a poly(A) tail. Random nonamers may be used as universal, non-specific primers.
Aplicação
Random Nonamers are suitable for:
- First-strand cDNA synthesis
- Preparing cDNA libraries (with oligo (dT) primers
- Prepare labeled DNA probes for hybridizations
Características e benefícios
- When preparing cDNA libraries or dealing with situations where specific primers are not suitable, the random nonamers can be combined with anchored oligo (dT)23 primers (Product No. O4387).
- In cases of incomplete or missing sequence information, or when specific primers are not effective, the random nonamers offer a solution for cDNA library preparation.
- The random nonamers′ priming capability at higher temperatures (up to 65°C) is reduced, ensuring that they do not interfere with polymerase chain reaction(PCR) following transcription.
- Randomnonamers can also be used to prepare labeled DNA probes for hybridizationstudiesRandom nonamers serve as alternatives to specific reverse transcription (RT) primers for first-strand synthesis, cDNA library construction, and other applications.
Componentes
Random Nonamers are provided as a 50 uM solution in water.
Outras notas
For laboratory use only. Not for drug, household, or other uses.
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Nº do produto
Descrição
Preços
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Oligonucleotide primers have been used to generate a cDNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones has enabled us to complete the viral RNA sequence and to study its variability within a viral
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Journal of neurochemistry, 111(3), 808-818 (2009-08-29)
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E M Brooks et al.
BioTechniques, 19(5), 806-812 (1995-11-01)
The secondary structure in mRNA is essential for many processes, but it can present a technical problem in making full-length cDNA with reverse transcriptases. Furthermore, different reverse transcriptases have differing abilities to transcribe through regions with secondary structure, which can
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