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R7397

Sigma-Aldrich

ProtectRNA RNase Inhibitor 500× Concentrate

RNase inhibitor for in situ hybridization assays

Sinônimo(s):

Ribonuclease inhibitor ProtectRNA

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30 ML
R$ 1.323,00

R$ 1.323,00


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30 ML
R$ 1.323,00

About This Item

Código UNSPSC:
41106300
NACRES:
NA.52

R$ 1.323,00


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Formulário

liquid

temperatura de armazenamento

2-8°C

Descrição geral

Not recommended in systems where other enzymatic activity is required.

ProtectRNA ribonuclease(RNase) Inhibitor inhibits most nucleic acid binding enzymes such as RNase and DNase, reverse transcriptase, some RNA and DNA polymerases, protein synthesis enzymes, and several restriction endonucleases. In contrast, ProtectRNA does not interfere with DNA and RNA hybridizations. The action ofProtectRNA as a nuclease inhibitor can be used as an advantage for in situ hybridization. ProtectRNA is useful during all steps of in situ hybridization for tissue RNA protection from RNase degradation. It is recommended to add ProtectRNA to all aqueous solutions used, from as early as the deparaffinization step until the hybridization is completed.

Aplicação

A potent inhibitor of most nucleic acid binding enzymes, and thus useful as an RNase inhibitor. Especially useful when performing in situ hybridization. If it is added to all aqueous solutions used, it eliminates the need for special glassware washing and after-wash treatments. The 500x concentrate is economical; 2 ml treats 1,000 ml of solution. Not recommended in systems where other enzymatic activity is required.
ProtectRNA RNase Inhibitor 500× Concentrate has been used in:
  • RNA extraction[1]
  • tissue sectioning and staining for laser capture microscopy[2]
  • the isolation of blood vessels from hippocampi[3]
ProtectRNA RNase Inhibitor 500× Concentrate is an RNase inhibitor.[4][5][6]

Características e benefícios

  • The 500× concentrate is economical
  • Eliminates the need for special dishwashing and“after washing” cleaning and treatments.

Quantidade

2mL treats 1,000ml of solution.

Outras notas

This product is for R&D use only, not for drug, household, or other uses.

Informações legais

ProtectRNA is a trademark of Sigma-Aldrich Co. LLC

Pictogramas

FlameCorrosion

Palavra indicadora

Warning

Frases de perigo

Declarações de precaução

Classificações de perigo

Flam. Liq. 3 - Met. Corr. 1

Código de classe de armazenamento

3 - Flammable liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

140.0 °F - closed cup

Ponto de fulgor (°C)

60 °C - closed cup


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Questions

  1. Hello, I am wondering what the concentration in U/uL of this RNAse inhibitor is. U being units. Best, Charles

    1 answer
    1. Unfortunately, this product is not tested to determine the unit activity. The solution is performance tested with a recommended starting dilution factor of 1:500.

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