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P5899

Sigma-Aldrich

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt ≥300,000, powder, γ-irradiated, suitable for cell culture, BioXtra

Sinônimo(s):

PDL HBr

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About This Item

Número CAS:
25988-63-0
Número MDL:
MFCD00237230
Código UNSPSC:
12352202
eCl@ss:
32160406
ID de substância PubChem:
24898718
NACRES:
NA.75

Nome do produto

Poli-L-lisina, mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

fonte biológica

synthetic (organic)

Nível de qualidade

200

esterilidade

γ-irradiated

linha de produto

BioXtra

Formulário

lyophilized powder

peso molecular

≥300,000

embalagem

pkg of 5 mg

técnica(s)

cell culture | mammalian: suitable

cobertura de superfície

4 μg/cm2

Impurezas

<10% water (by Karl Fischer)

solubilidade

H2O: soluble 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow

Condições de expedição

ambient

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

chave InChI

WBSCNDJQPKSPII-KKUMJFAQSA-N

Procurando produtos similares? Visita Guia de comparação de produtos

Aplicação

Poly-L-lysine hydrobromide has been used to coat:
  • 2-well plates before seeding muscle cells in a research study on rainbow trout myotube formation
  • flat bottom 96-well plates for in vitro neuronal stem cell differentiation
  • T75 flasks for primary astrocytic cultures

Ações bioquímicas/fisiológicas

Poly-L-lysine serves as a coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.

Componentes

A poli-L-lisina é um polímero de aminoácido com carga positiva, com cerca de um HBr por resíduo de lisina. O hidrobrometo possibilita que a poli-L-lisina esteja em uma forma cristalina solúvel em água. Uma pequena quantidade do produto pode ser encontrada na estrutura beta, porque o HBr interfere na ligação de hidrogênio entre o grupo amina e o grupo carboxila ou as porções que contêm N ou O.

Atenção

As soluções estéreis são estáveis por até 2 anos se armazenadas a 2 ºC a 8 °C. Deve ser armazenado dessecado a -20 °C.

Nota de análise

Molecular weight based on viscosity. Also assayed by MALLS.

Outras notas

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
0000315801
0000315801
SLCL5836
SLCF0030
SLBT1729

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Gustavo Castro et al.
ISRN ophthalmology, 2013, 752161-752161 (2014-02-22)
Purpose. The purpose of this work was to evaluate the retinal integration and differentiation of neurospheres formed by stem cells and mouse neural progenitor cells injected intravitreally in mice eyes with retinal injury. Methods. Eight male C57BL mice, 8 weeks
Polylysine for skin regeneration: A review of recent advances and future perspectives
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Journal of Separation Science, 5(7) (2021)
Direct addition of poly-lysine or poly-ethylenimine to the medium: A simple alternative to plate pre-coating
Alexander Faussner, et al.
PLoS ONE, 17(7) (2022)
Sonja Stadler et al.
BMC cell biology, 5(1), 44-44 (2004-11-24)
Between cell divisions the chromatin fiber of each chromosome is restricted to a subvolume of the interphase cell nucleus called chromosome territory. The internal organization of these chromosome territories is still largely unknown. We compared the large-scale chromatin structure of
Poly-L-lysine Prevents Senescence and Augments Growth in Culturing Mesenchymal Stem Cells Ex Vivo
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BioMed Research International (2016)
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Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Protocolos

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

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