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P4929

Sigma-Aldrich

Poly(deoxyinosinic-deoxycytidylic) acid sodium salt

double-stranded alternating copolymer

Sinônimo(s):

Poly(dI-dC) • Poly(dI-dC) sodium salt

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5 UNITS
R$ 1.991,00
10 UNITS
R$ 2.772,00
25 UNITS
R$ 5.541,00

R$ 1.991,00

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5 UNITS
R$ 1.991,00
10 UNITS
R$ 2.772,00
25 UNITS
R$ 5.541,00

About This Item

Número CAS:
118578-37-3
Número MDL:
MFCD00131970
Código UNSPSC:
41106305
NACRES:
NA.51

R$ 1.991,00

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fonte biológica

synthetic (organic)

Nível de qualidade

200

Formulário

powder

temperatura de armazenamento

−20°C

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Aplicação

Poly(deoxyinosinic-deoxycytidylic) acid (Poly(dI-dC) • Poly(dI-dC)) is an alternating copolymer used as a DNA substrate for evaluation of DNA methytransfeases, such as DNA-methyltransferase 1 and as double-stranded DNA model for conformational studies of DNA structure dynamics and drug, small molecule, interactions.
Poly(deoxyinosinic-deoxycytidylic) acid sodium salt has been used in a study that synthesized and characterized bis(2-(pyrimidin-2-γl)ethoxy)alkanes and their pharmacological activity. Poly(deoxyinosinic-deoxycytidylic) acid sodium salt has also been used in a study that investigated the growth of calcium carbonate films on LB/LbL matrices.
Poly(deoxyinosinic-deoxycytidylic) acid sodium salt has been used in the electrophoretic mobility shift assay in p65 protein[1] and neuro-2A protein lysate.[2] It has also been used as a substrate in the protein arginine methyltransferase 1 (PRMT1) and DNA methyltransferase 1 (DNMT1) selectivity assay.[3]

Ações bioquímicas/fisiológicas

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate.[4] At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt.[5] It is used in electrophoretic mobility shift assay (EMSA).[6]

Outras notas

Double-stranded alternating copolymer

Definição da unidade

One unit will yield an A260 of 1.0 in 1.0 ml of 20 mM sodium phosphate/100 mM NaCl, pH 7.0 (1 cm light path)

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
0000425632
0000425632
0000402757
0000389077
0000387689

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Dimitrios Priftis et al.
Langmuir : the ACS journal of surfaces and colloids, 28(23), 8721-8729 (2012-05-15)
A systematic study of the interfacial energy (γ) of polypeptide complex coacervates in aqueous solution was performed using a surface forces apparatus (SFA). Poly(L-lysine hydrochloride) (PLys) and poly(L-glutamic acid sodium salt) (PGA) were investigated as a model pair of oppositely
Meaghan L Clark et al.
Inorganic chemistry, 47(20), 9410-9418 (2008-09-25)
This paper focuses on DNA-binding interactions exhibited by Pt(dma-T)CN(+), where dma-T denotes 4'-dimethylamino-2,2':6',2''-terpyridine, and includes complementary studies of the corresponding pyrr-T complex, where pyrr-T denotes 4'-(N-pyrrolidinyl)-2,2':6',2''-terpyridine. The chromophores are useful for understanding the interesting and rather intricate DNA-binding interactions exhibited
Chao Bi et al.
Plant molecular biology, 94(1-2), 197-213 (2017-04-10)
It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present study, we showed that inhibition of the catalase activity with 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of Col-0, abi5 mutants and
Dexamethasone treatment of calves latently infected with bovine herpesvirus 1 leads to activation of the bICP0 early promoter, in part by the cellular transcription factor C/EBP-alpha
Workman A, et al.
Journal of Virology, 83(17), 8800-8809 (2009)
Identification of Selective, Cell Active Inhibitors of Protein Arginine Methyltransferase 5 through Structure-Based Virtual Screening and Biological Assays
Ye F, et al.
Journal of Chemical Information and Modeling, 58(5), 1066-1073 (2018)
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Questions

1–7 of 7 Questions  

  1. How to prepare the product of P4929-5U to 1ug/ul? What's the weight (mg), and molecular weight (g/mol) of this product?

    1 answer

    1. The polymer size of this material varies from lot to lot. The typical low range is 150 to 550 base pairs and the typical high range is 3000 to 5000 base pairs. The bulk of the product ranges from 1200 to 3000 base pairs. The molecular weight of a single Poly(dI-dC) base pair is 639.41 g/mol, resulting in an approximate molecular weight range of 767.2 kDa – 1,918.2 kDa for most of the compound. The unit per milligram value may range from 8 – 14 units per milligram and the vial content may range from 5.0 – 7.5 units. The specific vial content is lot specific and may range from 535 – 625 ug per vial. To prepare a 1 ug/ul solution, please see the link below to review the lot specific Certificate of Analysis:
      https://www.sigmaaldrich.com/product/sigma/p4929#product-documentation

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  2. Should I heat the product P4929-25U after resuspension to make aliquots? If yes, then how long and at what temperature? Thank you!

    1 answer

    1. This product is soluble in water or neutral buffer. Solubility is no longer considered part of the specifications, but it was previously reported to be soluble at 20 mg/mL in 0.1 M Tris-HCl, pH 8.8. Heat is not necessary to solubilize the product; dissolving in an appropriate solvent is the recommended method for resuspension.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. Is Is Product P4929,  Poly(deoxyinosinic-deoxycytidylic) acid sodium salt (Poly(dI-dC), stable in solution?

    1 answer

    1. Both the solution and solid are very stable and can be stored for extended periods (years) at -20 °C.

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  5. How many milligrams of Product P4929, Poly(deoxyinosinic-deoxycytidylic) acid sodium salt, do I have?

    1 answer

    1. The specifications for the product is approximately 13 U/mg, but the exact amount is lot specific.  One unit will yield an A260 of 1.0 in 1.0ml of water.  For example, the 5 unit size has approximately 0.384 mg.

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  6. What is the molecular weight of Product P4929, Poly(deoxyinosinic-deoxycytidylic) acid sodium salt?

    1 answer

    1. Molecular weight: Polymer size (by agarose gel electrophoresis) is lot dependent with a lower range of less than 150 to 550 base pairs and a high range of 3000 to over 5000 base pairs. Most of the material is between 1200 to 3000 base pairs.

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  7. What is the solubility of Is Product P4929,  Poly(deoxyinosinic-deoxycytidylic) acid sodium salt (Poly(dI-dC)?

    1 answer

    1. This product is soluble in water or neutral buffer at 20 mg/ml.

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