81349
Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt
free from low molecular weight nucleotides, lyophilized
Sinônimo(s):
Poly(dI-dC) sodium salt
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About This Item
Preço e disponibilidade não estão disponíveis no momento.
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![Poly[d(I-C)] lyophilized, pkg of 10 U (10108812001 [A<sub>260</sub> units]), pkg of 50 U (11219847001 [A<sub>260</sub> units])](/deepweb/assets/sigmaaldrich/product/images/352/091/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1/640/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1.jpg)
grau
for molecular biology
Nível de qualidade
Formulário
powder
qualidade
lyophilized
peso molecular
Mr ~2.5 × 106 (broad molecular weight distribution)
Mr ~2500000
embalagem
ampule of ≥10 units A260
temperatura de armazenamento
−20°C
Descrição geral
Poly (2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt is an alternating copolymer that acts as a substrate for DNA methyltransferases.
Aplicação
Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt has been used in electrophoretic mobility shift assay (EMSA) of the transcription factor AP-2 Alpha (TFAP2A)[1] and in southwestern blotting of human embryonic kidney cells (HEK293) nuclear extract.[2]
Suitable for
- non-specific blocking agent in EMSA
- evaluation of DNA methyltransferases
- DNA-small molecule or drug interaction studies
Ações bioquímicas/fisiológicas
Poly(2′-deoxyinosinic-2′-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate.[3] At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt.[4] It is used as a non-specific competitor in electrophoretic mobility shift assay (EMSA).[5]
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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An enzymatic method for the determination of dCTP and dGTP in picomole amounts.
L Skoog
European journal of biochemistry, 17(2), 202-208 (1970-12-01)
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Jia Y, et al.
Journal of Chromatography A, 1217(45), 7177-7181 (2010)
TFAP2A is a component of the ZEB1/2 network that regulates TGFB1-induced epithelial to mesenchymal transition
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Optimization of Competitor Poly (dI-dC) Poly (dI-dC) Levels is Advised in DNA-Protein Interaction Studies Involving Enriched Nuclear Proteins
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Biotechniques, 20(3), 439-444 (1996)
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