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NDEGLY

Sigma-Aldrich

Native Protein Deglycosylation Kit

Sinônimo(s):

Protein Deglycosylation Kit

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1 KIT
R$ 4.948,00

R$ 4.948,00

Previsão de entrega em16 de abril de 2025

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1 KIT
R$ 4.948,00

About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

R$ 4.948,00

Previsão de entrega em16 de abril de 2025

conjugado

(N-linked)

Nível de qualidade

200

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Descrição geral

All complex oligosaccharides can be reduced to the trimannosylchitobiose core by treatment of the glycoproteins with neuraminidase, β-galactosidase, and N-acetylglucosaminidase. Fucosidases may be required in some situations. The remaining trimannosylchitobiose core structures can be removed with endoglycosidase F3. Biantennary and triantennary structures can be immediately removed by endoglycosidases F2 and F3, respectively. Oligomannose and hybrid structures can be removed by Endoglycosidase F1.
For more information on each type of endoglycosidase, please refer to the Bulletin.
The Native Protein Deglycosylation is designed for the deglycoslylation of N-linked oligosaccharides from PNGase F-resistant native proteins. Endoglycosidases F1, F2, and F3 are less sensitive to protein conformation than PNGase F and are more suitable for removal of all classes of N-linked oligosaccharides without protein denaturation.

Aplicação

Native Protein Deglycosylation Kit has been for N-deglycosylation of various enzymes such as laccase,[1] tomato nuclease TBN1 and endo-β-1,3-glucanase.[2]

Armazenamento e estabilidade

The NDEGLY Kit ships on wet ice and storage at 2–8 °C is recommended. This kit may be used for at least 1 year when stored as indicated. Several days exposure to ambient temperatures will not reduce the activity of the enzymes in this kit.

Somente componentes do kit

Nº do produto
Descrição
  • Endoglycosidase F1 .3 U
  • Endoglycosidase F2 .1 U
  • Endoglycosidase F3 .1 U
  • Endoglycosidase F1 reaction buffer 200 μL
  • Endoglycosidase F2 & 3 reaction buffer 200 μL

Código de classe de armazenamento

12 - Non Combustible Liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
SLCN2040
SLCP3048
SLCP1406
SLCL9476
SLCK9717

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Visite a Biblioteca de Documentos

Laccase isoform diversity in basidiomycete Lentinus strigosus 1566: Potential for phenylpropanoid polymerization
Kolomytseva MP, et al.
International Journal of Biological Macromolecules (2019)
Flavio Mena et al.
Neuroendocrinology, 91(1), 77-93 (2009-07-11)
We have previously shown that soluble factor(s) in conditioned media (CM) from the central and peripheral regions of the anterior pituitary (AP) gland of lactating rats promoted the in vitro dose-related release of prolactin (PRL) from pituitary glands of male
Junyi Ma et al.
Molecular pharmacology, 69(4), 1137-1145 (2006-01-10)
Opioid analgesics remain the choice for the treatment of moderate to severe pain. Recent research has established that the mu-opioid receptor is predominantly responsible for mediating many opioid actions, including analgesia and opioid tolerance. However, the function of delta-opioid receptors
Adela Rodríguez-Romero et al.
Acta crystallographica. Section D, Biological crystallography, 70(Pt 2), 329-341 (2014-02-18)
Endogenous glycosylated Hev b 2 (endo-β-1,3-glucanase) from Hevea brasiliensis is an important latex allergen that is recognized by IgE antibodies from patients who suffer from latex allergy. The carbohydrate moieties of Hev b 2 constitute a potentially important IgE-binding epitope
Amir E Zeituni et al.
Journal of bacteriology, 192(16), 4103-4110 (2010-06-22)
We recently reported that the oral mucosal pathogen Porphyromonas gingivalis, through its 67-kDa Mfa1 (minor) fimbria, targets the C-type lectin receptor DC-SIGN for invasion and persistence within human monocyte-derived dendritic cells (DCs). The DCs respond by inducing an immunosuppressive and
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