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PP0201

Sigma-Aldrich

Glycoprofile™ II, Enzymatic In-Solution N-Deglycosylation Kit

sufficient for 20 samples

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.26

temperatura de armazenamento

2-8°C

Descrição geral

Contains sufficient reagents for a minimum of 20 reactions when the sample size is between one to two mg of a typical glycoprotein.

Aplicação

GlycoProfile II Enzymatic In-Solution N-Deglycosylation Kit was used to study the effects of glycosylation of the N-acetyl-D-galactosamine (GalNAc)-specific lectin found in Schizophyllum commune.
GlycoProfile™ II has been optimized to provide a convenient and reproducible method to remove N-linked glycans from glycoproteins and is compatible with subsequent MALDI-TOF mass spectrometric analysis without interference from any of the reaction components.
Glycoprofile™ II, Enzymatic In-Solution N-Deglycosylation Kit has been used in the deglycosylation of Schizophyllum commune lectin, Arabidopsis mannanase, human umbilical vein endothelial cells (HUVEC) protein lysate and organic solute transporter protein, Ostβ.

Informações legais

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Somente componentes do kit

Nº do produto
Descrição
  • 10× Reaction Buffer 1 mL/vial

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS
  • P7367PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE), for proteomics 50 USDS
  • R7884Ribonuclease B from bovine pancreas, BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE) .5 mgSDS
  • O9882Octyl β-D-glucopyranoside, BioXtra, ≥98% (GC) 100 mgSDS
  • M31482-Mercaptoethanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) .9 mLSDS

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Órgãos-alvo

Liver,Heart

Código de classe de armazenamento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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GlycoProfile™ Glycan Clean-Up Cartridge

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G4548

GlycoProfile™ Glycan Clean-Up Cartridge

PNGase F lyophilized powder, recombinant, expressed in E. coli

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F8435

PNGase F

PNGase F from Elizabethkingia miricola buffered aqueous solution

Sigma-Aldrich

G5166

PNGase F from Elizabethkingia miricola

N-Glycosidase F PNGase F of Flavobacterium meningosepticum, recombinant from E. coli

Roche

NGLYF-RO

N-Glycosidase F

2-Mercaptoetanol for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Sigma-Aldrich

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2-Mercaptoetanol

Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown
Haarmann A, et al.
PLoS ONE, 5(10), e13568-e13568 (2010)
Podjana Chumkhunthod et al.
Biochimica et biophysica acta, 1760(3), 326-332 (2006-03-02)
An N-acetyl-D-galactosamine (GalNAc)-specific lectin was purified from the edible mushroom, Schizophyllum commune, using affinity chromatography on a porcine stomach mucin (PSM)-Sepharose 4B column. Under reducing and non-reducing conditions, SDS-polyacrylamide gel electrophoresis gave a major band of 31.5 kDa. The Schizophyllum
Purification and characterization of an N-acetyl-D-galactosamine-specific lectin from the edible mushroom Schizophyllum commune
Chumkhunthod P, et al.
Biochim. Biophys. Acta Gen. Subj., 1760(3), 326-332 (2006)
Elena Schwagerus et al.
Pflugers Archiv : European journal of physiology, 467(11), 2257-2273 (2015-02-14)
Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human
Christen Y L Yuen et al.
Plant & cell physiology, 58(6), 1103-1117 (2017-04-27)
Members of the protein disulfide isomerase (PDI)-C subfamily are chimeric proteins containing the thioredoxin (Trx) domain of PDIs, and the conserved N- and C-terminal Pfam domains of Erv41p/Erv46p-type cargo receptors. They are unique to plants and chromalveolates. The Arabidopsis genome
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