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N3162

Sigma-Aldrich

Anti-NBS1 (Nibrin) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinônimo(s):

Anti-p95 Protein of the MRE11/RAD50 Complex

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About This Item

Número MDL:
MFCD07784851
Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

rabbit

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

forma

buffered aqueous solution

peso molecular

antigen 95 kDa (also recognizes 100 kDa band)

reatividade de espécies

canine, human, rat, chimpanzee

técnica(s)

immunoprecipitation (IP): 5-10 μg
indirect immunofluorescence: 5-10 μg/mL using Hela cells, fixed with paraformaldehyde/triton
microarray: suitable
western blot: 1-2 μg/mL using MCF7 nuclear extracts

nº de adesão UniProt

O60934

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... NBN(4683)
mouse ... Nbn(27354)
rat ... Nbn(85482)

Descrição geral

NBS1 (Nibrin), also known as p95 protein of the meiotic recombination 11 homolog 1/DNA repair protein RAD50 (MRE11/RAD50) complex. NBS1 contains two domains found in the cell cycle checkpoint proteins forkhead-associated domain (FHA) and an adjacent breast cancer carboxyterminal domain (BRCT). NBS1 is one of the protein components of the double-strand break repair complex NBS1/MRE11/p50.
Nibrin (NBS1) is made up of 754 amino acids.

Imunogênio

synthetic peptide corresponding to amino acids 692-706 of mouse NBS1 (nibrin), conjugated to KLH via an N-terminal added cysteine residue.

Aplicação

Anti-NBS1 (Nibrin) antibody has been used:
  • in immunofluorence
  • in immunoblotting
  • in immunoprecipitation

Ações bioquímicas/fisiológicas

NBS1 (Nibrin) was first isolated as a protein involved in DNA repair through analysis of mutations in patients with Nijmegen breakage syndrome (NBS). The majority of NBS patients bear a five base pairs deletion that leads to truncated NBS1, called 657del5. p95/NBS1 (nibrin) deficiency abrogates the formation of the MRE11/RAD50 ionizing radiation-induced foci, revealing a molecular link between DSB repair and cell cycle checkpoints activated by DNA damage.
Nibrin (NBS1) is involved in stabilizing genomes and has a role in development of cancers. It also takes part in the repair mechanism after double strand breaks in the DNA.

forma física

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Adel Alblihy et al.
Biomedicines, 9(1) (2021-01-14)
Platinum resistance seriously impacts on the survival outcomes of patients with ovarian cancers. Platinum-induced DNA damage is processed through DNA repair. NBS1 is a key DNA repair protein. Here, we evaluated the role of NBS1 in ovarian cancers. NBS1 expression
Nibrin, a novel DNA double-strand break repair protein, is mutated in Nijmegen breakage syndrome
Varon R, et al.
Cell, 93(3), 467-476 (1998)
VRK1 phosphorylates and protects NBS1 from ubiquitination and proteasomal degradation in response to DNA damage
Monsalve D M, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1863(4), 760-769 (2016)
Jana Suchánková et al.
Biology of the cell, 107(12), 440-454 (2015-10-21)
The DNA damage response is a fundamental, well-regulated process that occurs in the genome to recognise DNA lesions. Here, we studied kinetics of proteins involved in DNA repair pathways and their recruitment to DNA lesions during the cell cycle. In
Kelly Gray et al.
Circulation research, 116(5), 816-826 (2014-12-20)
DNA damage and the DNA damage response have been identified in human atherosclerosis, including in vascular smooth muscle cells (VSMCs). However, although double-stranded breaks (DSBs) are hypothesized to promote plaque progression and instability, in part, by promoting cell senescence, apoptosis
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