Pular para o conteúdo
Merck
Todas as fotos(2)

Documentos

MAK155

Sigma-Aldrich

Fluorometric Sphingomyelinase Assay Kit

sufficient for 200 fluorometric tests (red fluorescence)

Sinônimo(s):

Neutral Sphingomyelinase Assay Kit

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(2)

About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 200 fluorometric tests (red fluorescence)

método de detecção

fluorometric

doença(s) relevante(s)

cancer; rheumatological diseases

temperatura de armazenamento

−20°C

Informações sobre genes

human ... SMPD2(6610) , SMPD3(55512) , SMPD4(55627)
mouse ... SMPD2(20598) , SMPD3(58994) , SMPD4(77626)
rat ... SMPD2(83537) , SMPD3(94338) , SMPD4(303790)

Descrição geral

Sphingomyelinase (SMase), or sphingomyelin phosphodiesterase, catalyzes the hydrolytic cleavage of sphingolipid to phosphocholine and ceramide, a second messenger involved in multiple cellular responses including the cellular response to stress. There are at least five types of SMases identified, which are classified according to their cation dependence, cellular location, and pH optima for activity, and include the acidic and neutral sphingomyelinase. Neutral SMases, along with the acidic SMases, are thought to be major contributors to the production of ceramide in response to cellular stress. Mutations in is associated with metabolic disorders like Niemann-Pick disease B.

Adequação

This kit is suitable for the detection of sphingomyelin in a variety of samples such as blood and cell extracts.

Princípio

The Sphingomyelinase Assay Kit provides a simple and direct procedure for measuring neutral SMase activity in a variety of samples such as blood or cell extracts, or for screening SMase activators and inhibitors. SMase activity is determined by a coupled enzyme assay, which results in a fluorescent product (λex =540/λem = 590 nm) proportional to the SMase activity present.

Palavra indicadora

Danger

Frases de perigo

H302,H317,H318,H334,H410

Classificações de perigo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1

Código de classe de armazenamento

10 - Combustible liquids

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Sphingomyelinases: enzymology and membrane activity.
Go?i F M and Alonso A
Febs Letters, 531(1), 38-46 (2002)
Sphingomyelinases: their regulation and roles in cardiovascular pathophysiology.
Pavoine C and Pecker F
Cardiovascular Research, 82(2), 175-183 (2009)
Secretory sphingomyelinase in health and disease.
Kornhuber J, et al.
Biological Chemistry, 396(6-7), 707-736 (2015)

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica