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M0535

Sigma-Aldrich

Mycoplasma Broth

powder, suitable for microbiology

Sinônimo(s):

Mycoplasma media

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About This Item

Código UNSPSC:
41106200
NACRES:
NA.74

forma

powder

Nível de qualidade

200

técnica(s)

microbiological culture: suitable

solubilidade

H2O: 25.5 g/L (Autoclave at 121°C, 15 psi for 15 minutes. Store prepared medium at 0-5°C.)

aplicação(ões)

microbiology

Categorias relacionadas

Descrição geral

Mycoplasma Broth is a basal medium which, after enrichment with yeast extract, horse serum, and antibiotics, will support the growth of Mycoplasma sp. The components contained in this basal medium do not exhibit inhibitory or toxic effects when used to culture mycoplasma. A special mineral supplement has been added to improve the growth and colony characteristics of the organism.

Aplicação

Mycoplasma Broth has been used for isolation and cultivation of mycoplasma. It may also be used to grow Porphyromonas gingivalis under anerobic conditions.

Componentes

Powder contains (g/L): 10.0 bacterial peptone, 10.0 LAB-LEMCO powder, 5.0 sodium chloride, 0.5 mineral supplement.

Nota de preparo

Basal Medium:

1. Suspend 25.5 g of powdered medium in 1L of distilled water. Stir until the powder dissolves.

2. Adjust the pH of the solution to 7.8 ± 0.2.

3. Autoclave at 121 °C and 15 psi for 15 minutes to sterilize. Cool to 50 °C and add sterile supplements.

Armazenamento e estabilidade

Store dry powdered medium at room temperature and prepare medium at 2-8 °C.

Outras notas

The product is intended for laboratory use only.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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