L4025

Liticase

lyophilized powder, ≥200 units/mg solid

• Sinônimo(s): (1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase, Bacterial lyticase, Lysing enzyme
• Número CAS: 37340-57-1
• Número MDL: MFCD00131560
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• fonte biológica: bacterial (Arthrobacter luteus)
• Nível de qualidade: 200
• Formulário: lyophilized powder
• atividade específica: ≥200 units/mg solid
• técnica(s): cell based assay: suitable
• adequação: suitable for cell lysis
• aplicação(ões): diagnostic assay manufacturing
• temperatura de armazenamento: −20°C

Descrição

Aplicação

Lyticase from Arthrobacter luteus has been used for resuspending cells to extract high-molecular weight DNA for polymerase chain reaction (PCR) fingerprint analyses.

Lyticase from Arthrobacter luteus has been used:

• as a component in spheroplasting buffer to prepare yeast nuclear extracts[1]
• for digestion in sample preparation for β-glucan enzymatic assay using yeast[1]
• to lyse cells for RNA isolation[2]

Ações bioquímicas/fisiológicas

A liticase hidrolisa a poli-β(1→3)-glicose, como glucana da parede celular de levedura.

Lyticase enzyme is frequently used in fungal research, particularly for species identification using polymerase chain reaction (PCR)-based techniques. It can break down β (1→3) and β (1→4) bonds between glucose units.

Lyticase is a lysing enzyme that is used to extract DNA from yeast cells by inducing partial spheroplast formation.[3] Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast, which are difficult to disrupt because the cell walls may form capsules or resistant spores. Lyticase contains β-(1→;3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities.[4] It is useful with yeast cells such as e.g. Candida,[5] Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.

Definição da unidade

Uma unidade produzirá uma ΔA₈₀₀ de 0,001 por minuto em pH 7,5 a 25 °C, usando uma suspensão de levedura como substrato em uma mistura de reação de 3 ml.

Outras notas

Veja mais informações sobre enzimas para análise de carboidratos complexos em www.sigma-aldrich.com/enzymeexplorer

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Declarações de precaução: P261 - P284 - P501
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)