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L2762

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O26:B6

purified by gel-filtration chromatography

Sinônimo(s):

LPS

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About This Item

Número CE:
297-473-0
Número MDL:
MFCD00164401
Código UNSPSC:
12352201
NACRES:
NA.25

fonte biológica

Escherichia coli (O26:B6)

Nível de qualidade

200

forma

lyophilized powder

purificado por

gel-filtration chromatography

Impurezas

<5% Protein

cor

white to faint yellow

solubilidade

water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

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Descrição geral

This product is extracted from E. coli serotype O26:B6 and purified by gel filtration. The source strain is ATCC 12795. LPS from serotype 026:B6 is the only LPS from E. coli which exhibits short chain-length behavior on SDS-PAGE. The short chain-length of this LPS is closer to that of the mutant rough strain LPS. This LPS serotype has been used at 1 μg/ml to stimulate neutrophils.

Aplicação

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Ações bioquímicas/fisiológicas

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Nota de preparo

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Outras notas

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Pictogramas

Skull and crossbones
GHS06

Palavra indicadora

Danger

Frases de perigo

H300

Declarações de precaução

P264 - P270 - P301 + P310 - P405 - P501

Classificações de perigo

Acute Tox. 2 Oral

Código de classe de armazenamento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Randy Tatt Yhew Haw et al.
Journal of neuroinflammation, 11, 134-134 (2014-07-31)
We report a novel method of culturing microglia in three dimension (3D) using collagen as a substrate. By culturing microglia within a matrix, we aim to emulate the physical state of microglia embedded within parenchyma. BV2 microglia cell suspensions were
Catherine H Hackett et al.
Stem cells and development, 20(4), 721-735 (2010-08-21)
A long-term goal of mesenchymal progenitor cell (MPC) research is to identify cell-surface markers to facilitate MPC isolation. One reported MPC feature in humans and other species is lack of CD14 (lipopolysaccharide receptor) expression. The aim of this study was
Xiaobo Wu et al.
Journal of immunology (Baltimore, Md. : 1950), 200(8), 2786-2797 (2018-03-14)
Factor D (FD) is an essential component of the complement alternative pathway (AP). It is an attractive pharmaceutical target because it is an AP-specific protease circulating in blood. Most components of the complement activation pathways are produced by the liver
Yosra Bedoui et al.
PLoS neglected tropical diseases, 12(8), e0006634-e0006634 (2018-08-04)
Chikungunya virus (CHIKV) is a mosquito-transmitted RNA alphavirus causing major outbreaks of infectious chronic inflammatory rheumatisms (CIR). Recently, methotrexate (MTX), a disease modifying anti-rheumatic drug has been used successfully to treat patients suffering from rheumatoid-like arthritis post-CHIK but its immunomodulatory
Shinsmon Jose et al.
Journal of neuroinflammation, 11, 149-149 (2014-09-04)
Progression of neurodegenerative diseases occurs when microglia, upon persistent activation, perpetuate a cycle of damage in the central nervous system. Use of mesenchymal stem cells (MSC) has been suggested as an approach to manage microglia activation based on their immunomodulatory
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Conteúdo relacionado

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

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