H7523

HEPES

BioXtra, pH 5.0-6.5 (1 M in H₂O), ≥99.5% (titration)

• Sinônimo(s): Ácido 4-(2-hidroxietil)piperazina-1-etanossulfônico, Ácido N-(2-hidroxietil)piperazina-N′-2-etanossulfônico
• Fórmula empírica (Notação de Hill): C₈H₁₈N₂O₄S
• Número CAS: 7365-45-9
• Peso molecular: 238.30
• Beilstein: 883043
• Número CE: 230-907-9
• Número MDL: MFCD00006158
• Código UNSPSC: 12161700
• ID de substância PubChem: 24895796
• NACRES: NA.25

Propriedades

• linha de produto: BioXtra
• Nível de qualidade: 400
• Ensaio: ≥99.5% (titration)
• Formulário: powder
• Impurezas: Insoluble matter, passes filter test
• resíduo de ignição (900 °C): ≤0.1% (as SO₄)
• perda: ≤1.0% loss on drying, 110°C
• pH: 5.0-6.5 (1 M in H₂O)
• faixa de pH útil: 6.8-8.2
• pKa (25 °C): 7.5
• solubilidade: H₂O: 1 M, clear, colorless
• traços de ânion: chloride (Cl^-): ≤0.05%
• traços de cátion: Al: ≤0.0005%; As: ≤0.0001%; Ba: ≤0.0005%; Bi: ≤0.0005%; Ca: ≤0.001%; Cd: ≤0.0005%; Co: ≤0.0005%; Cr: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Li: ≤0.0005%; Mg: ≤0.0005%; Mn: ≤0.0005%; Mo: ≤0.0005%; Na: ≤0.005%; Ni: ≤0.0005%; Pb: ≤0.0005%; Sr: ≤0.0005%; Zn: ≤0.0005%
• Absorção: ≤0.035 at 280 in H₂O at 1 M; ≤0.045 at 260 in H₂O at 1 M
• aplicação(ões): diagnostic assay manufacturing
• cadeia de caracteres SMILES: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• chave InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Descrição

Descrição geral

HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]

Aplicação

HEPES has been used:

• As a component of homogenisation buffer used in protein sample preparation[3]
• To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation[4]
• As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation[5]
• As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.[6][7]

Outras notas

Easily compare specifications for HEPES products with the HEPES specification table.

Informações de segurança

• Código de classe de armazenamento: 13 - Non Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)