H6147

HEPES

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

• Sinônimo(s): Ácido 4-(2-hidroxietil)piperazina-1-etanossulfônico, Ácido N-(2-hidroxietil)piperazina-N′-2-etanossulfônico
• Fórmula empírica (Notação de Hill): C₈H₁₈N₂O₄S
• Número CAS: 7365-45-9
• Peso molecular: 238.30
• Beilstein: 883043
• Número CE: 230-907-9
• Número MDL: MFCD00006158
• Código UNSPSC: 12161704
• ID de substância PubChem: 24895731
• NACRES: NA.75

Propriedades

• linha de produto: BioXtra
• Nível de qualidade: 400
• Ensaio: ≥99.5% (titration)
• Formulário: powder
• técnica(s): cell culture | embryo: suitable
• pH: 5.0-6.5 (25 °C, 238 g/L)
• faixa de pH útil: 6.8-8.2
• pKa (25 °C): 7.5; 7.5
• solubilidade: water: 500 mg/mL, clear, colorless
• Absorção: ≤0.05 at 290 in H₂O at 33%
• cadeia de caracteres SMILES: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• chave InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Descrição

Descrição geral

HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2] It is typically used in cell culture at concentration between 5mM to 30mM.

Aplicação

HEPES has been used:

• As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells[3]
• As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production[4]
• in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation[5]

Outras notas

This product has been qualified for use in cell and mouse embryo culture.

Easily compare specifications for HEPES products with the HEPES specification table.

propriedades físicas

Useful pH range: 6.8 - 8.2

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)