GERPN2251
cAMP Biotrak™ EIA, (Non-Acetylation Protocol)
Cytiva RPN2251
Sinônimo(s):
cAMP Enzyme Immunoassay Kit
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About This Item
Código UNSPSC:
41116126
NACRES:
NA.32
Produtos recomendados
fabricante/nome comercial
Cytiva RPN2251
embalagem
pkg of 96 wells
temperatura de armazenamento
2-8°C
Descrição geral
EIAs and ELISAs are convenient, high-sensitivity, peroxidase-based technologies that enable the amount of protein present in a sample to be measured in a single day. Novel lysis reagents have been developed (Lysis Reagents 1 and 2) to facilitate simple and rapid extraction of cellular molecules.
The Biotrak™ cAMP competitive enzymeimmunoassay system is specifically designed for research purposes. The kit includes protocols using novel lysis reagents in order to facilitate simple and rapid extraction of cAMP from cell cultures. These components avoid the requirement for traditional, time-consuming extraction procedures and obviate the need for removal of extraction reagents prior to measurement. It combines the use of a peroxidase-labeled cAMP conjugate, a specific antiserum which can be immobilized on to precoated microplates, and a one-pot stabilized substrate solution.
Lysis reagent 1 hydrolyzes cell membranes to release intra-cellular
cAMP. Lysis Reagent 2 sequesters the key component in Lysis
Reagent 1 and ensures cAMP is free for subsequent analysis. The
detergent/sequestrant complex does not interfere with antigen:
antibody binding. Lysis Reagent 1 is simply added to cultured cells,
followed by a 5 to 10 min incubation before assay. The
antiserum is reconstituted with Lysis Reagent 2. The assay is based
on competition between unlabeled cAMP and a fixed quantity of
peroxidase-labeled cAMP, for a limited number of binding sites on a
cAMP specific antibody.
The Biotrak™ cAMP competitive enzymeimmunoassay system is specifically designed for research purposes. The kit includes protocols using novel lysis reagents in order to facilitate simple and rapid extraction of cAMP from cell cultures. These components avoid the requirement for traditional, time-consuming extraction procedures and obviate the need for removal of extraction reagents prior to measurement. It combines the use of a peroxidase-labeled cAMP conjugate, a specific antiserum which can be immobilized on to precoated microplates, and a one-pot stabilized substrate solution.
Lysis reagent 1 hydrolyzes cell membranes to release intra-cellular
cAMP. Lysis Reagent 2 sequesters the key component in Lysis
Reagent 1 and ensures cAMP is free for subsequent analysis. The
detergent/sequestrant complex does not interfere with antigen:
antibody binding. Lysis Reagent 1 is simply added to cultured cells,
followed by a 5 to 10 min incubation before assay. The
antiserum is reconstituted with Lysis Reagent 2. The assay is based
on competition between unlabeled cAMP and a fixed quantity of
peroxidase-labeled cAMP, for a limited number of binding sites on a
cAMP specific antibody.
Aplicação
Características e benefícios
- Elimination of inconvenient, time-consuming extraction
procedures. - Flexible method-choice of assay protocols.
- Range 25 - 6400 fmol/well (non-acetylation protocol).
- Rapid assay protocol.
- Nonradioactive.
- Specific for cAMP.
- Precise and accurate measurement
- Ready-to-use substrate.
- Colour coded reagents
Armazenamento e estabilidade
Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Nota de análise
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Informações legais
Biotrak is a trademark of Cytiva
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Código de classe de armazenamento
13 - Non Combustible Solids
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