GE28-9893-35

HiLoad^® 16/600 Superdex^® 200 pg

Cytiva 28-9893-35, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

• Sinônimo(s): 16/600 Superdex 200, HiLoad Superdex 200
• Código UNSPSC: 41115700
• NACRES: SB.52

Propriedades

• Materiais: bed support (polyamide); glass column; glass fiber (polypropylene reinforced)
• peso molecular: 10000-600000 Da (globular proteins)
• embalagem: pkg of 1 ea
• fabricante/nome comercial: Cytiva 28-9893-35
• Parâmetros: <1.0 mL/min flow rate; <5 mL sample volume; 0.3 Mpa (42 psi)
• volume do leito: 120 mL
• coluna C × D.I.: 60 cm × 16 mm
• matriz: agarose dextran composite; dextran-agarose particle platform; polymeric
• Grupo ativo da matriz: dextran-agarose phase
• Tamanho médio da partícula: 34 μm
• cleaning: 1-14
• working range: 3-12
• técnica de separação: size exclusion (SEC)

Descrição

Descrição geral

HiLoad^® 16/600 Superdex^® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice for separating MAbs from dimers and contaminants.

Superdex^® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex^® 30 pg), Mr 3 000 to 70 000 (Superdex^® 75 pg), and Mr 10 000 600 000 (Superdex^® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Características e benefícios

• High resolution with short run times and good recovery.
• High capacity at high flow rates.
• Separations can be scaled up to production levels.
• Prepacked for convenience and reproducibility.
• 1/16” fittings for convenient connections to AKTA design systems.

Adequação

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

for high-resolution preparative gel filtration chromatography, e.g, separate mAbs from dimers and contaminants

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Informações legais

HiLoad is a registered trademark of Cytiva

Superdex is a registered trademark of Cytiva

Informações de segurança

• Pictogramas: GHS02
• Palavra indicadora: Warning
• Frases de perigo: H226
• Declarações de precaução: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de classe de armazenamento: 3 - Flammable liquids