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GE28-9670-70

Protein G Mag Sepharose Xtra

Cytiva 28-9670-70, pack of 5 × 1 mL

Sinônimo(s):

Protein G magnetic beads

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

protein G

embalagem

pack of 5 × 1 mL

fabricante/nome comercial

Cytiva 28-9670-70

condição de armazenamento

(20% Ethanol, 10% medium slurry)

Parâmetros

Room temperature temp. range

matriz

paramagnetic, spherical, highly cross-linked agarose particles

capacidade

27 mg binding capacity(human IgG/ml gel)

temperatura de armazenamento

2-8°C

Descrição geral

protein G Mag Sepharose Xtra magnetic beads are designed for rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants. The high capacity, > 27 mg human IgG /mL medium allow for the efficient capture of the antibody with high yield and purity. Approximately 350 μg purified human IgG can be obtained from each purification run.

Aplicação

Protein G Mag Sepharose Xtra magnetic beads combine proven affinity chromatography methods with the magnetic bead platform to improve simplification and ease-of-use in sample preparation. Mag Sepharose beads are dense, black and visible, and this makes them simple to handle. Less smearing effects and aggregate formations of the magnetic beads mean that no detergents are needed in the buffers.

Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device. Protein G Mag Sepharose Xtra is available in a 2 × 1 mL pack for 20 purifications and in a 5 × 1 mL pack for 50 purifications. The product is based on Sepharose medium with magnetite incorporated and the ligand is native Protein G. Protein G has high affinity for the Fc region of IgG antibody from a variety of species.


Protein G Mag Sepharose is another product in the Mag Sepharose platform which is designed for the rapid capture and enrichment of Protein samples from cell lysates and biological fluids using immunoprecipitation technology. Protein G Mag Sepharose is made with an optimized ligand for immunoprecipitation.

Características e benefícios

  • Efficient, high capacity small-scale purification and screening of antibodies from various species; 27 μg human IgG/μL magnetic beads
  • High purity and yield
  • Robust parallel screening of antibodies with high reproducibility
  • Simple capture of antibodies from small or large sample volumes (low-microliter to high-milliliter scale)
  • Visible and dense Sepharose based magnetic beads give increased simplicity for handling
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Artigos

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Protocolos

Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra enable high-capacity small-scale antibody purification and screening.

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