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GE17-5073-01

SP Sepharose™ XL

Cytiva 17-5073-01, pack of 300 mL

Sinônimo(s):

Cation Exchange Resin, SP Sepharose XL

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

sulphopropyl

descrição

Ion Exchanger Type (value)

embalagem

pack of 300 mL

fabricante/nome comercial

Cytiva 17-5073-01

matriz

6% cross-linked agarose with dextran surface extenders

tamanho de partícula

45-165 μm

diâmetro médio

90 μm

cleaning in place

3-14

working range

4-13

adequação

suitable for bioprocess medium

Categorias relacionadas

Descrição geral

SP Sepharose™ XL is a strong cation exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.

Aplicação

SP Sepharose™ XL is based on a highly cross-linked, bead formed 6% Agarose matrix similar to the well established SP Sepharose™ Fast FLow media. Dextran chains are covalently coupled to the Agarose matrix and modified with sulphopropyl (SP) strong cation exchange groups through chemically stable ether bonds. The stable Agarose matrix and long, flexible chains of Dextran with bound charged groups work together to increase loading capacity whilst allowing high flow rates


With its high loading capacities and excellent physical and chemical stability, SP Sepharose™ XL is suitable for the capture stage of a downstream process, which is characterised by large loads of crude samples.


As member of the BioProcess media range, SP Sepharose™ XL meets industrial demands with security of supply and comprehensive technical and regulatory support.

Características e benefícios

  • High loading capacities give more productive capture from clarified feedstocks.
  • Strong cation exchanger designed for capture steps
  • High chemical stability for effective CIP/sanitization
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Sepharose is a trademark of Cytiva

Pictogramas

Flame
GHS02

Palavra indicadora

Warning

Frases de perigo

H226

Código de classe de armazenamento

3 - Flammable liquids

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Artigos

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocolos

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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