GE17-1279-02
rProtein A Sepharose™ Fast Flow
Cytiva 17-1279-02, pack of 25 mL
About This Item
Produtos recomendados
ligand
recombinant protein A (E. coli)
embalagem
pack of 25 mL
fabricante/nome comercial
Cytiva 17-1279-02
condição de armazenamento
(20% Ehtanol)
Matriz
4% cross-linked agarose
tamanho de partícula
60-165 μm
diâmetro médio
90 μm (d50v)
cleaning in place
2-11
Faixa de trabalho
3-10
capacidade
~50 mg binding capacity (human lgG/ml medium)
adequação
suitable for bioprocess medium
temperatura de armazenamento
2-8°C
Categorias relacionadas
Descrição geral
Monoclonal and polyclonal antibody purification
rProtein A Sepharose 4 Fast Flow is built upon the well-established Sepharose Fast Flow platform and is used in many approved monoclonal antibody processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix.The highly crosslinked agarose resin enables high flow rates and high productivity.
Protein A resin characteristics
The recombinant protein is produced in E. coli and engineered with an oriented coupling to enhance binding capacity. Epoxy-based coupling ensures low ligand leakage. Specificity of the recombinant protein for the Fc region of IgG is similar to the native protein, giving a high degree of selectivity and yield.
Streamlined scale up
Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.
Características e benefícios
- For monoclonal and polyclonal antibody purification at both laboratory and process scale.
- Well established among Protein A resins as excellent for monoclonal antibody (mAb) processes.
- Free from animal-derived components.
- Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
- Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Armazenamento e estabilidade
Nota de análise
Informações legais
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Código de classe de armazenamento
3 - Flammable liquids
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Certificados de análise (COA)
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Protocolos
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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