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Documentos Principais

GE17-0507-01

Sigma-Aldrich

Mono S® HR 16/10

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About This Item

Código UNSPSC:
41115700
NACRES:
SB.52

ligand

-OCH2CH(OH)CH2OCH2CH(OH)CH2SO3-

Materiais

PEEK column

descrição

Ion Exchanger Type (Methyl sulfonate strong cation)

embalagem

pkg of 1 ea

Parâmetros

435 psi

tamanho do leito

16 mm × 100 mm

volume do leito

20 mL

D.I. da coluna

16 mm

tamanho

Matriz

polymeric

Grupo ativo da matriz

methyl sulfonate phase

Tamanho médio da partícula

10 μm

cleaning

1-14

Faixa de trabalho

2-12

técnica de separação

cation exchange

Descrição geral

Mono S® HR 16/10 is a strong cation exchanger prepacked with MonoBeads. The column is an excellent choice for the polishing step in purification of proteins, peptides, and other biomolecules when high purity is required. The 20 mL bed volume of the column offer high capacity.

Aplicação

  • Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
  • Compatible with ÄKTA® design, FPLC™ System, and other high-performance LC systems.
  • MonoBeads are also available as Mono P® for chromatofocusing

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Outras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

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Informações legais

FPLC is a trademark of Cytiva
Mono P is a registered trademark of Cytiva
Mono S is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictogramas

Flame
GHS02

Palavra indicadora

Warning

Frases de perigo

H226

Código de classe de armazenamento

3 - Flammable liquids

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Certificados de análise (COA)

Lot/Batch Number

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Artigos

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocolos

MonoBeads: Purification of Milligram Quantities with Highest Resolution

This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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