E5160

Epidermal Growth Factor from mouse

≥90% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell cutlure

• Sinônimo(s): Mouse EGF, Mouse Epidermal Growth Factor
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• Nome do produto: Epidermal Growth Factor from mouse, EGF
• fonte biológica: mouse
• Nível de qualidade: 200
• recombinante: expressed in E. coli
• Ensaio: ≥90% (SDS-PAGE)
• Formulário: lyophilized powder
• potência: 0.05-1 ng/mL ED₅₀/EC₅₀
• peso molecular: ~6 kDa
• embalagem: pkg of 100 μg
• condição de armazenamento: avoid repeated freeze/thaw cycles
• técnica(s): cell culture | mammalian: suitable
• Impurezas: ≤1 EU/μg endotoxin (Protein)
• cor: white
• solubilidade: water: soluble 0.10 mL, clear, colorless
• nº de adesão UniProt: P01132
• temperatura de armazenamento: −20°C
• Informações sobre genes: mouse ... Egf(13645)

Descrição

Descrição geral

Epidermal Growth Factor (EGF) is a small mitogenic polypeptide (∼6kDa) present in many mammalian species and distributed throughout a wide number of tissues and body fluids.[1] Four ErbB (HER) family receptor tyrosine kinases, including EGFR/ErbB1, ErbB2, ErbB3, and ErbB4 mediate responses to EGF family members.[2] Human and mouse EGFs are very similar, but not identical in their physical and chemical properties. Of the 53 amino acid residues comprising each of the two polypeptides, 37 are common to both molecules, and three disulfide bonds are formed in the same relative positions.[3]

Aplicação

Epidermal Growth Factor from mouse has been used as a basal (N2B27) medium supplement for culturing differentiated mouse embryonic stem (E14) cells.[4]

Ações bioquímicas/fisiológicas

EGF (epidermal growth factor) is involved in cellular proliferation, differentiation, and survival.[5] Moreover, it was found to affect various biological activities like angiogenesis, inhibition of gastric acid secretion, modulation of the synthesis of a number of hormones, synthesis and turn-over of proteins of the extracellular matrix, calcium release from bone tissue (thus promoting bone resorption), chemoattraction of fibroblasts and epithelial cells, and alone or in combination with other cytokines, mediation of wound healing processes.[6][7][8][9][10][11][12] EGF is mitogenic for a large variety cell types, including fibroblasts, epithelial cells, endothelial cells, chondrocytes, and SV40-3T3 cells.[3]

forma física

The product is lyophilized from a 0.2 μm-filtered solution of phosphate buffered saline (PBS), pH 7.4.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1