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DUO92105

Sigma-Aldrich

Duolink® In Situ Red Starter Kit Goat/Rabbit

Sinônimo(s):

in situ Proximity Ligation Assay Kit, Protein Protein Interaction Kit

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About This Item

Código UNSPSC:
41116158
NACRES:
NA.32

linha de produto

Duolink®

técnica(s)

proximity ligation assay: suitable

fluorescência

λex 594 nm; λem 624 nm (red) (Texas Red®; Zeiss Filter set 31)

adequação

suitable for fluorescence

Categorias relacionadas

Aplicação

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Goat PLUS and Anti-Rabbit MINUS), red detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink® In Situ Red Starter Kit Goat/Rabbit requires one primary antibody from goat and one primary antibody from rabbit. Red fluorescence detection reagents are often used with Texas Red® filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

View full Duolink® product list

Características e benefícios

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Informações legais

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
Texas Red is a registered trademark of Life Technologies

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS
  • DUO92005Duolink® In Situ PLA® Probe Anti-Rabbit MINUSSDS
  • DUO92003Duolink® In Situ PLA® Probe Anti-Goat PLUSSDS
  • DUO92008Duolink® In Situ Detection Reagents RedSDS
  • DUO82040Duolink® In Situ Mounting Medium with DAPISDS
  • DUO82049Duolink® In Situ Wash Buffers, FluorescenceSDS

Pictogramas

Health hazardCorrosion
GHS08,GHS05

Palavra indicadora

Danger

Frases de perigo

H290,H334,H412

Declarações de precaução

P234 - P261 - P273 - P284 - P390 - P501

Classificações de perigo

Aquatic Chronic 3 - Met. Corr. 1 - Resp. Sens. 1

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Gunisha Sagar et al.
Gut, 65(7), 1165-1174 (2015-06-11)
New-onset diabetes and concomitant weight loss occurring several months before the clinical presentation of pancreatic cancer (PC) appear to be paraneoplastic phenomena caused by tumour-secreted products. Our recent findings have shown exosomal adrenomedullin (AM) is important in development of diabetes
Shaida Ouladan et al.
International journal of oncology, 46(6), 2595-2605 (2015-04-23)
Solitary fibrous tumors (SFTs) are rare mesenchymal neoplasms, displaying variable morphological and clinicopathological features. Supportive immunohistochemical markers such as CD34, CD99, BCL2 and LSD1 are commonly applied in the differential diagnosis of SFTs, although none is sufficiently sensitive or specific
Dipti Pravin Lambade et al.
Journal of clinical and diagnostic research : JCDR, 9(2), ZC01-ZC05 (2015-04-11)
The purpose of this vitro study was to comparatively evaluate the adhesive bonding of dual cured resin luting agents with lithium disilicate ceramic material. Porcelain laminate veneers were prepared with lithium disilicate ceramic material i.e. IPS Empress II( E-Max Press).
Linbo Wang et al.
Journal of cancer research and clinical oncology, 140(12), 1997-2008 (2014-07-10)
Our previous work identified leucine zipper transcription factor-like 1 (LZTFL1) as a novel tumor suppressor gene, with its expression correlated with survival outcome in gastric cancer (GC) patients. This study focuses on the role of LZTFL1 in GC aggression and
Peng Jiang et al.
PloS one, 10(6), e0128744-e0128744 (2015-06-09)
Exposure of platelets to collagen triggers the formation of a platelet clot. Pharmacological agents capable of inhibiting platelet activation by collagen are thus of potential therapeutic interest. Thrombus formation is initiated by the interaction of the GPIb-V-IX complex with collagen-bound
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Artigos

Duolink® PLA Technology for Neuroscience Applications

Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.

How to Optimize the Duolink® Proximity Ligation Assay

Things to consider for preparation, setup and execution of the Duolink® assay protocol

Duolink® PLA Troubleshooting Guide

Support information including tips and tricks, frequently asked questions, and basic troubleshooting.

Protocolos

Duolink® In Situ Short Instructions - Fluorescence

This page details the Duolink® In Situ Short Protocol for fluorescence detection

Duolink® PLA Fluorescence Protocol

This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.

Conteúdo relacionado

Duolink® PLA Applications

Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay

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