D6944

Anti-Dopamine Transporter (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinônimo(s): Anti-DAT, Anti-DAT1, Anti-SLC6A3, Anti-Solute carrier family 6, member 3
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen ~50 kDa
• reatividade de espécies: mouse (predicted), rat, human
• validação aprimorada: recombinant expression; Learn more about Antibody Enhanced Validation
• concentração: ~1.0 mg/mL
• técnica(s): immunofluorescence: 5-10 μg/mL using SH-SY-5Y cells; western blot: 0.5-1 μg/mL using rat brain tissue extract
• nº de adesão UniProt: Q01959
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... SLC6A3(6531) ; mouse ... Slc6a3(13162) ; rat ... Slc6a3(24898)

Descrição

Descrição geral

Dopamine transporter belongs to a family of biogenic amine transporters that include the norepinephrine, serotonin (SERT), and vesicular monoamine transporters. These transporters are members of a larger Na⁺/Cl^--dependent neurotransmitter transporter family.

Solute carrier family 6 member 3 (SLC6A3) is mapped to human chromosome 5p15.33. It is also known as the dopamine transporter (DAT) and has a zinc binding site and 12 transmembrane region.

Imunogênio

synthetic peptide corresponding to amino acids 42-59 of rat dopamine transporter conjugated to KLH. This sequence is highly conserved in mouse DAT (single amino acid substitution) and has lower homology (72% identity) to human DAT.

Aplicação

Anti-Dopamine Transporter antibody produced in rabbit is suitable for western blot analysis at a working concentration of 0.5-1μg/mL using rat brain extract (cytosolic S1 fraction) and HEK-293T cells expressing human DAT1. It was used as a primary antibody in western analysis for dopamine transporter in several studies.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Ações bioquímicas/fisiológicas

DAT regulates dopaminergic-neurotransmission. It is localized on presynaptic nerve terminals and mediates the active reuptake of dopamine from the synapse, thus terminating the neurotransmitter signal. It is the primary site for the action of neurotoxins, such as MPTP, and the drugs of abuse, cocaine and amphetamine. It has been implicated in a number of dopamine-related disorders, including attention deficit hyperactivity disorder (ADHD), bipolar disorder, clinical depression, and alcoholism. DAT may also have a role in selective vulnerability of nigrostriatal dopaminergic neurons in Parkinson′s disease as it can transport dopamine neurotoxins.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Outras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)