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D1878

Sigma-Aldrich

2,6-Dichloroindophenol sodium salt hydrate

Powder, BioReagent

Sinônimo(s):

2,6-Dichloro-N-(4-hydroxyphenyl)-1,4-benzoquinoneimine sodium salt, 2,6-Dichlorobenzenone-indophenol sodium salt, 2,6-Dichlorophenolindophenol sodium salt hydrate, DCIP, DPIP

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5 G
R$ 705,00
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R$ 2.456,00

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5 G
R$ 705,00
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R$ 2.456,00

About This Item

Fórmula empírica (Notação de Hill):
C12H6Cl2NNaO2 · xH2O
Número CAS:
1266615-56-8
Peso molecular:
290.08 (anhydrous basis)
Beilstein:
3641229
Número CE:
210-640-4
Número MDL:
MFCD00150014
Código UNSPSC:
12171500
ID de substância PubChem:
57654075
NACRES:
NA.47

R$ 705,00

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Nome do produto

2,6-Dichloroindophenol sodium salt hydrate, suitable for vitamin C determination, BioReagent

linha de produto

BioReagent

Nível de qualidade

200

Formulário

powder

solubilidade

H2O: soluble 10 mg/mL, clear, blue to very deep blue

adequação

suitable for vitamin C determination

aplicação(ões)

diagnostic assay manufacturing
food and beverages
hematology
histology

temperatura de armazenamento

room temp

cadeia de caracteres SMILES

O.[Na+].[O-]c1ccc(cc1)\N=C2/C=C(Cl)C(=O)C(Cl)=C2

InChI

1S/C12H7Cl2NO2.Na.H2O/c13-10-5-8(6-11(14)12(10)17)15-7-1-3-9(16)4-2-7;;/h1-6,16H;;1H2/q;+1;/p-1

chave InChI

XHSOLXWCGCVQHE-UHFFFAOYSA-M

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Descrição geral

2,6-Dichloroindophenol sodium salt hydrate is a blue redox dye.

Aplicação

2,6-Dichloroindophenol sodium salt hydrate is suitable for vitamin C determination. 2,6-Dichloroindophenol (DCIP) may be used in the following studies:
  • As redox dye to investigate the efficiency of gold nanoparticles (Au-NP) for enzymatic activity of glucose oxidase (GOx).[1]
  • To compose UVB specific dosimeter.[2]
  • Quantification of vitamin C (L-ascorbic acid) in fruit juices.[3]
  • As substrate in NQO1 assay to investigate the liver cytosolic NQO1 activity spectrophotometrically.[4]
  • Mitochondrial succinate CoQ oxidoreductase (complex II) activity assay.[5]

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
BCCK3015
BCCH8797
BCCD6459
BCCD6463
BCCD6464

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Spectrophotometric evaluation of gold nanoparticles as red-ox mediator for glucose oxidase.
Ramanaviciene A, et al.
Sensors and Actuators B, Chemical, 137(2), 483-489 (2009)
Andrew Mills et al.
The Analyst, 134(5), 845-850 (2009-04-22)
A UVB specific dosimeter is described comprising: a redox dye (2,6-dichloroindophenol, DCIP), a semiconductor (tin(IV) oxide, SnO(2)) and a sacrificial electron donor (glycerol) dispersed in a polymer (hydroxy ethyl cellulose, HEC) film. The dosimeter is blue in the absence of
Misty Prince et al.
Toxicology letters, 185(3), 180-186 (2009-01-20)
Naturally occurring coumarins possess anti-carcinogenic activities in part by inducing carcinogen-detoxifying enzymes glutathione S-transferase (GST) and/or NAD(P)H quinone oxidoreductase (NQO1). Our goal was to determine whether citrus coumarins induce hepatic GST and/or NQO1 via activation of Nrf2 and the antioxidant
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Questions

1–8 of 8 Questions  

  1. Has Sigma Aldrich performed solution stability studies for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?

    1 answer

    1. No. Unfortunately, we have not performed solution stability studies on this product.

      Helpful?

  2. Has Product D1878, 2,6-Dichloroindophenol sodium salt hydrate, been used as a pH or redox indicator?

    1 answer

    1. Yes. Conn's Biological Stains indicates Dichloroindophenol sodium salt in acid is  red and blue in alkali. The product is colorless when reduced.

      Helpful?

  3. Other than use as a reagent for determination of Vitamin C, are there other reported uses of Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?

    1 answer

    1. Yes. Conn's Biological Stains indicates Dichloroindophenol sodium salt has been used as an electron receptor, for use in demonstrating NADPH diaphorase and to stain glutathione reductase electrophoresis gels.

      Helpful?

  4. Are there published references for Dichloroindophenol sodium salt being used for the determination of Vitamin C?

    1 answer

    1. Yes. Please see: W. Horowitz ed. Official Methods of Analysis of the AOAC Washington D.C. , 777, (1970) and  K. Hiromi et al., Anal. Biochem. 101, 421, (1980).

      Helpful?

  5. Are there substances known to interfere in Vitamin C determination?

    1 answer

    1. Yes. Interfering substances are ferrous, stannous, and cuprous sulfite or thiosulfate ions, and SO2.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. What is the solubility for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?

    1 answer

    1. During quality control testing, Sigma Aldrich tests solubility at 10 mg/mL in water.  Conn's Biological Stains, 10th Edition states that dichloroindophenol sodium salt is soluble up to 3% in water and up to 2% in ethanol.

      Helpful?

  8. Are the absorbance maxima and the extinction coefficients available for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?

    1 answer

    1. Our Analytical Department has run absorbance spectra on many lots of D1878. Using 0.1 M phosphate buffer, pH 8.3 as the solvent, absorbance maxima in the range of 600-605 nm and a mM extinction coefficient of 18.0-19.7 have been reported.

      Helpful?

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